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  • 1 Yangtze University, China
  • | 2 University of Agriculture, Faisalabad, Pakistan
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Numerous studies showed that lipid transfer proteins (LTPs) play important roles in flower, development, cuticular wax deposition and pathogen responses; however, their roles in abiotic stresses are relatively less reported. This study characterized the function of a maize LTP gene (ZmLTP3) during drought stress. ZmLTP3 gene was transferred into maize inbred line Jing2416; subsequently the glyphosate and drought tolerance of the over-expression (OE) lines were analyzed. Analysis showed that OE lines could significantly enhance drought tolerance. Transgenic maize lines OE6, OE7 and OE8 showed lower cell membrane damage, higher chlorophyll contents, higher protective enzymes activities, better growth and development under drought condition. The results strongly indicated that overexpression of ZmLTP3 could increase drought tolerances in maize.

  • Cameron, K.D., Teece, M.A., Smart, L.B. 2006. Increased accumulation of cuticular wax and expression of lipid transfer protein in response to periodic drying events in leaves of tree tobacco. Plant Physiol. 140:176183.

    • Search Google Scholar
    • Export Citation
  • Canevascini, S., Caderas, D., Mandel, T., Fleming, A., Dupuis, I., Kuhlemeier, C. 1996. Tissue-specific expression and promoter analysis of the tobacco Ltp1 gene. Plant Physiol. 112:513524.

    • Search Google Scholar
    • Export Citation
  • Castro, M.S., Gerhardt, I.R., Orru, S., Pucci, P., Bloch, C. 2003. Purification and characterization of a small (7.3 kDa) putative lipid transfer protein from maize seeds. J. Chromatogr. B. 794:109114.

    • Search Google Scholar
    • Export Citation
  • Debono, A., Yeats, T.H., Rose, J.K., Bird, D., Jetter, R., Kunst, L., Samuels, L. 2009. Arabidopsis LTPG is a glycosylphosphatidylinositol-anchored lipid transfer protein required for export of lipids to the plant surface. Plant Cell. 21:12301238.

    • Search Google Scholar
    • Export Citation
  • Edstam, M.M., Viitanen, L., Salminen, T.A., Edqvist, J. 2011. Evolutionary history of the non-specific lipid transfer proteins. Mol. Plant. 4:947964.

    • Search Google Scholar
    • Export Citation
  • Edstam, M.M., Laurila, M., Höglund, A., Raman, A., Dahlström, K.M., Salminen, T.A., Edqvist, J., Blomqvist, K. 2014. Characterization of the GPI-anchored lipid transfer proteins in the moss Physcomitrella patens. Plant Physiol. Biochem. 75:5569.

    • Search Google Scholar
    • Export Citation
  • Fan, Y., Du, K., Gao, Y., Kong, Y., Chu, C., Sokolovc, V., Wang, Y. 2013. Transformation of LTP gene into Brassica napus to enhance its resistance to Sclerotinia sclerotiorum. Russ. J. Genet+. 49:380387.

    • Search Google Scholar
    • Export Citation
  • Garcia-Garrido, J.M., Menossi, M., Puigdimen, P., Martinez-Izquierdo, J.A., Delseny, M. 1998. Characterization of a gene encoding an abscissic acid inducible type 2 lipid transfer protein from rice. FEBS Lett. 428: 193199.

    • Search Google Scholar
    • Export Citation
  • Huang, M.D., Chen, T.L., Huang, A.H. 2013. Abundant type III lipid transfer proteins in Arabidopsis tapetum are secreted to the locule and become a constituent of the pollen exine. Plant physiol. 163:12181229.

    • Search Google Scholar
    • Export Citation
  • Iraki, N.M., Singh, N.K., Bressan, R.A., Carpita, N.C. 1989. Cell walls of tobacco cells and changes in composition associated with reduced growth upon adaptation to water and saline stress. Plant Physiol. 91:4853.

    • Search Google Scholar
    • Export Citation
  • Kader, J.C. 1996. Lipid-transfer proteins in plants. Annu. Rev. Plant Phys. 47:627654.

  • Kader, J.C. 1997. Lipid transfer proteins: a puzzling family of plant proteins. Trends Plant Sci. 2:6670.

  • Kader, J.C., Julienne, M., Vergnolle, C. 1984. Purification and characterization of a spinach-leaf protein capable of transferring phospholipids from liposomes to mitochondria or chloroplasts. Eur. J. Biochem. 139:411416.

    • Search Google Scholar
    • Export Citation
  • Kirubakaran, S.I., Begum, S.M., Ulaganathan, K., Sakthivel, N. 2008. Characterization of a new antifungal lipid transfer protein from wheat. Plant Physiol. Bioch. 46:918927.

    • Search Google Scholar
    • Export Citation
  • Kumar, P., Tewari, R.K., Sharma, P.N. 2008. Modulation of copper toxicity-induced oxidative damage by excess supply of iron in maize plants. Plant Cell Rep. 27:399409.

    • Search Google Scholar
    • Export Citation
  • Lee, S.B., Go, Y.S., Bae, H.J., Park, J.H., Cho, S.H., Cho, H.J., Lee, D.S., Park, O.K., Hwang, I., Suh, M.C. 2009. Disruption of glycosylphosphatidylinositol-anchored lipid transfer protein gene altered cuticular lipid composition, increased plastoglobules, and enhanced susceptibility to infection by the fungal pathogen Alternaria brassicicola. Plant Physiol. 150:4254.

    • Search Google Scholar
    • Export Citation
  • Li, J., Guo, X.W., Zhang, Z.B, Sun, H.J., Zou, H.W., Luo, C., Huang, C.L., Yu, R., Wu, Z.Y. 2014. Studies on transferring ATNCED3 into maize inbred line. Crops. 1:5862.

    • Search Google Scholar
    • Export Citation
  • Mittova, V., Guy, M., Ta, M., Volokita, M. 2004. Salinity up-regulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii. J. Exp. Bot. 55: 11051113.

    • Search Google Scholar
    • Export Citation
  • Noctor, G., Foyer, C.H. 1998. Simultaneous measurement of foliar glutathione, gamma-glutamylcysteine, and amino acids by high performance liquid chromatography: comparison with two other assay methods for glutathione. Anal. Biochem. 264:98110.

    • Search Google Scholar
    • Export Citation
  • Nonami, H., Boyer, J.S. 1990. Primary events regulating stem growth at low water potentials. Plant Physiol. 93:16011609.

  • Patkar, R.N., Chattoo, B.B. 2006. Transgenic indica rice expressing nsLTP like protein shows enhanced resistance to both fungal and bacterial pathogens. Mol. Breeding. 17:159171.

    • Search Google Scholar
    • Export Citation
  • Sterk, P., Booij, H., Schellekens, G.A., Van Kammen, A., De Vries, S.C. 1991. Cell-specific expression of the carrot EP2 lipid transfer protein gene. Plant Cell. 3:907921.

    • Search Google Scholar
    • Export Citation
  • Suelves, M., Puigdomenech, P. 1997. Different lipid transfer protein mRNA accumulates in distinct parts of Prunus amygdalus flower. Plant Sci. 129:4956.

    • Search Google Scholar
    • Export Citation
  • Sun, X.Y., Zhu, Y., Zhao, M.M., Li, Z.X., Zou, H.W. 2014. Cloning and characterization of a lipid transfer protein gene, ZmLTP3, from maize. J. Maize Sci. 22:6266.

    • Search Google Scholar
    • Export Citation
  • Trevor, H.Y., Jocelyn, K.C. 2008. The biochemistry and biology of extracellular plant lipid transfer proteins (LTPs). Protein Sci. 17:191198.

    • Search Google Scholar
    • Export Citation
  • Zou, H.W., Wang, X.H., Huang, C.L., Chen, J.S., Zhang, X.H., Luo, C., Yu, R., Wu, Z.Y. 2014. Stress-inducible expression of a gene encoding C-repeat binding factor 4 (CBF4) from Arabidopsis improved performance of transgenic maize under drought condition. Plant Omics J. 7:94101.

    • Search Google Scholar
    • Export Citation