Virus-induced gene silencing of TaERECTA increases stomatal density in bread wheat

J.C. Zheng; T. Liu; J.Q. Li; X. Wang; W.Y. Li; F. Xu; Q.W. Zhan

doi:10.1556/0806.46.2018.054

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Cereal Research Communications

Volume/Issue:: Volume 47: Issue 1

Virus-induced gene silencing of TaERECTA increases stomatal density in bread wheat

Authors:

J.C. Zheng

J.C. Zheng Anhui Science and Technology University, Feng yang, Anhui, 233100, China

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T. Liu

T. Liu Anhui Science and Technology University, Feng yang, Anhui, 233100, China

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J.Q. Li

J.Q. Li Anhui Science and Technology University, Feng yang, Anhui, 233100, China

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X. Wang

X. Wang Anhui Science and Technology University, Feng yang, Anhui, 233100, China

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W.Y. Li

W.Y. Li Anhui Science and Technology University, Feng yang, Anhui, 233100, China

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F. Xu

F. Xu Anhui Science and Technology University, Feng yang, Anhui, 233100, China

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Q.W. Zhan

Q.W. Zhan Anhui Science and Technology University, Feng yang, Anhui, 233100, China

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Pages:: 67–77

Online Publication Date:: Mar 2019

Publication Date:: 01 Mar 2019

Article Category:: Research Article

DOI:: https://doi.org/10.1556/0806.46.2018.054

Keywords:: BSMV-VIGS; stomatal conductance; stomatal density; TaERECTA ; Triticum aestivum L.

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Barley stripe mosaic virus (BSMV)-based virus induced gene silencing (VIGS) is an effective strategy for rapid determination of functional genes in wheat plants. ERECTA genes are reported to regulate stomatal pattern of plants, and manipulation of TaERECTA (a homologue of ERECTA in bread wheat) is a potential route for investigating stomatal development. Here, the leucine-rich repeat domains (LRRs) and transmembrane domains of TaERECTA were selected to gain BSMV:ER-LR and BSMV:ER-TM constructs, respectively, targeting TaERECTA for silencing in wheat cultivars ‘Bobwhite’ and ‘Cadenza’, to identify the function of TaERECTA on stomatal patterns. The results showed that reduced expression of TaERECTA caused an increased stomatal and epidermal cell density by average 13.5% and 3.3%, respectively, due to the significantly reduced size of leaf epidermal and stomatal cells, and this led to an increase in stomatal conductance. These suggest that modulation of TaERECTA offers further opportunities in stomatal engineering for the adaptation of photosynthesis in wheat.

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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

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Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)