Winter wheat samples infected by eyespot were collected during the years 2015–2017. In total 143 Oculimacula spp. isolates were obtained and determined by PCR. Oculimacula yallundae isolates prevailed (122) over Oculimacula acuformis isolates (12) and 9 mixed isolates of both species. O. acuformis predominated only on one locality. The reaction of 104 Oculimacula spp. isolates to ten different fungicides was evaluated in laboratory tests on Petri dishes. The highest efficacy showed the combination of epoxiconazole + fluxapyroxad + pyraclostrobin and the lowest efficacy was evaluated after application of prothioconazole + trifloxystrobin. The decrease of efficacy of fungicide no. 6 (prothioconazole + trifloxystrobin) has been observed since 2015. The sensitivity of 114 Oculimacula spp. isolates to prochloraz has been tested. More than half of the isolates (58%) showed low to medium resistance. One isolate of O. yallundae from the year 2017 showed high resistance to prochloraz (ED50 ranged 1.7 μg ∙ ml–1). The rest of the isolates (42%) were sensitive to prochloraz.
Avenot, H.F., Michailide, T.J. 2010. Progress in understanding molecular mechanisms and evolution of resistance to succinate dehydrogenase inhibiting (SDHI) fungicides in phytopathogenic fungi. Crop Prot. 29(7):643–651.
Bierman, S.M., Fitt, B.D.L., van den Bosch, F., Bateman, G.L., Jenkyn, J.F., Welham, S.J. 2002. Changes in populations of the eyespot fungi Tapesia yallundae and T. acuformis under different fungicide regimes in successive crops of winter wheat, 1984–2000. Plant Pathol. 51:191–201.
Dumalasová, V., Palicová, J., Hanzalová, A., Bížová, I., Leišová-Svobodová, L. 2015. Eyespot resistance gene Pch1 and methods of study of its effectiveness in wheat cultivars. Czech J. Genet. Plant 51:166–173.
Dyer, P.W., Hansen, J., Delaney, A., Lucas, J.A. 2000. Genetic control of resistance to the sterol 14a-demethylase inhibitor fungicide prochloraz in the cereal eyespot pathogen Tapesia yallundae. Appl. Environ. Microb. 66(11):4599–4604.
Finney, D.J. 1971. Probit analysis (Third ed.). Cambridge, UK: Cambridge University Press.
Leroux, P., Gredt, M. 1997. Evolution of fungicide resistance in the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis in France. Pestic. Sci. 51:321–327.
Leroux, P., Gredt, M., Remuson, F., Micoud, A., Walker, A.-S. 2013. Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae. Pest Manag. Sci. 69:15–26.
Lucas, J.A., Dyer, P.S., Muray, T.D. 2000. Pathogenicity, host specificity, and population biology of Tapesia spp., causal agents of eyespot disease in cereals. Adv. Bot. Res. 33:226–258.
Matušinsky, P., Svacinová, I., Jonaviciene, A., Tvaružek, L. 2017. Long-term dynamics of causative agents of stem base diseases in winter wheat and reaction of Czech Oculimacula spp. and Microdochium spp. populations to prochloraz. Eur. J. Plant Pathol. 148:199–206.
Opalski, K.S., Tresch, S., Kogel, K.H., Grossmann, K., Kohle, H., Huckelhoven, R. 2006. Metrafenone: studies on the mode of action of a novel cereal powdery mildew fungicide. Pest Manag. Sci. 62(5):393–401.
Palicová, J., Matušinsky, P., Dumalasová, V., Hanzalová, A., Bížová, I. 2018. Resistance of winter wheat cultivars to eyespot and characterisation of causal agents of the disease. Plant Protect. Sci. 54(1):24–30.
Ramanauskiene, J., Gaurilcikiene, J. 2016. Incidence of eyespot in winter wheat and quantification of the fungi Oculimacula acuformis and O. yallundae in Lithuania. J. Plant Dis. Protect. 123(2):75–81.
Walsh, K., Korimbocus, J., Boonham, N., Jennings, P., Hims, M. 2005. Using real-time PCR to discriminate and quantify the closely related wheat pathogens Oculimacula yallundae and Oculimacula acuformis. J. Phytopathol. 153:715–721.
Wei, L., Muranty, H., Zhang, H. 2011. Advances and prospects in wheat eyespot research: contributions from genetics and molecular tools. J. Phytopathol. 159:457–470.