Cereal Research Communications
Volume/Issue:
Volume 47: Issue 2
DNA quantification to assess Zymoseptoria tritici on a susceptible cultivar of durum wheat to establish the best timing for fungicide application in an italian environment
Authors:
S. Tonti Alma Mater Studiorum University of Bologna, Bologna, Italy

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G. Alvisi Consorzio Agrario dell'Emilia, Research and Development, Test Facility, San Giorgio di Piano, Bologna, Italy

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A. Pisi Alma Mater Studiorum University of Bologna, Bologna, Italy

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P. Nipoti Alma Mater Studiorum University of Bologna, Bologna, Italy

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A. Prodi Alma Mater Studiorum University of Bologna, Bologna, Italy

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Pages:
304–313
Online Publication Date:
Jun 2019
Publication Date:
01 Jun 2019
Article Category:
Research Article
DOI:
https://doi.org/10.1556/0806.47.2019.03
Keywords:
durum wheat; Zymoseptoria tritici ; qPCR; fungicide
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Zymoseptoria tritici, a globally distributed pathogen, is responsible of Septoria tritici blotch (STB), one of the most damaging wheat diseases. In Italy the incidence of STB has increased during the past few years. The presence of Z. tritici on flag leaves of susceptible durum wheat plants, cultivar San Carlo, after a single artificial inoculation with two inoculum concentrations at different vegetative stages has been evaluated in the plain of Bologna (North of Italy), in a two year field study (2012–2013). The pathogen presence was also assessed in natural infection conditions after a fungicide application in the second year (2013). The results obtained, by visual examination (Incidence, Disease Severity) and DNA quantification by Real time PCR, demonstrated that BBCH 39 (flag leaf stage) is the most susceptible vegetative stage, independently of inoculum concentration and climatic conditions. A good correlation between Disease Severity and DNA quantity was observed in either sampling methods, entire flag leaves and flag leaf discs. Thereafter the most suitable period to obtain the best crop protection with only one fungicide treatment is the flag leaf stage.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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