Fumonisins were produced by six strains of Fusarium moniliforme and Fusarium proliferatum on inoculated rice culture. For study of fumonisins of the B series (B1, B2, B3, and B4) a quick and efficient two-step reversed-phase OPLC method was developed in which the first step serves for cleaning of the samples. Measurements were performed with the automatic OPLC instrument. Results showed that one isolate of F. moniliforme and one of F. proliferatum produced all the fumonisins investigated – the other isolates produced no fumonisins. This work is the first report of the separation of all the fumonisins of the B series by use of a planar layer liquid chromatographic technique.
A. Bottalico, A. Logrieco, and A. Visconti, in: J. Chelkowski (Ed.), Fusarium Mycotoxins, Taxonomy and Pathogenicity, Elsevier, Amsterdam, 1989.
W. C. A. Gelderblom, E. Semple, W. F. O. Marasas, and E. Farber, Carcinogenesis 13 (1992) 433.
St. M. Musser, M. L. Gay, and E.P. Mazzola, J. Nat. Prod. 59 (1996) 970.
G. E. Rottinghaus, C. E. Coatney, and H. C. Minor, J. Vet. Diagn. Invest. 4 (1992) 326.
E. Tyihák and E. Mincsovics, in: Sz. Nyiredy (Ed.), Planar Chromatography 2000, Proc Int Symp Planar Separations, Research Institute for Medicinal Plants, Budakalász, 2000.
E. Mincsovics, K. Ferenczi-Fodor, and E. Tyihák, in: J. Sherma and B. Fried (Eds), Handbook of Thin-Layer Chromatography, Marcel Dekker, New York, 1996.
E. Mincsovics, M. Garami, L. Kecskés, B. Tapa, Z. Végh, Gy. Kátay, and E. Tyihák, JAOAC Int. 82 (1999) 587.
H. Jork, W. Funk, W. Fisher, and H. Wimmer, in: Thin-Layer Chromatography, Volume 1a, VCH, Weinheim, 1990.