Planar chromatographic methods thin-layer chromatography (TLC) and over-pressure thin-layer chromatography (OPLC) have been developed for the determination of pigments in plant leaves. Plants with variegated leaves, which sometimes contain both anthocyanins and chlorophyll, are a reasonable material for investigation. Multicolored Coleus, Prunus cerasifera, and Rhus hirta leaves were taken for quantitative determination of anthocyanins and chlorophyll. The separation of pigments from plant leaves, which depends on compound polarity was optimized by appropriate selection of mobile and stationary phases and chromatographic conditions.
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