JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 15: Issue 3
HPTLC determination of lupeol in Grewia tiliaefolia
Authors:
Shrishailappa Badami J.S.S. College of Pharmacy Ootacamund 643001 TN India

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Mahesh Gupta J.S.S. College of Pharmacy Ootacamund 643001 TN India

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Noble Mathew J.S.S. College of Pharmacy Ootacamund 643001 TN India

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Subramania Meyyanathan J.S.S. College of Pharmacy Ootacamund 643001 TN India

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Bhojraj Suresh J.S.S. College of Pharmacy Ootacamund 643001 TN India

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David Bendell University of Sunderland School of Sciences Sunderland SR2 7EE UK

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Pages:
183–186
Online Publication Date:
08 Mar 2007
Publication Date:
01 Jun 2002
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.15.2002.3.4
Keywords:
HPTLC; Standardization; Grewia tiliaefolia ; Lupeol
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Among the complex mixture of biologically active compounds in the bark of Grewia tiliaefolia , a plant used in folklore, lupeol, a constituent of the bark, has been used as an analytical marker indicative of the quality of the plant. A sensitive and reliable quantitative high-performance thin-layer chromatographic method has been developed for the determination of lupeol from Grewia tiliaefolia . Chloroform extracts of bark from five different sources were used for HPTLC on silica gel with benzene-ethyl acetate, 95 + 5, as mobile phase. Under these conditions the RF of lupeol was 0.40. The calibration plot was linear in the range 0.5 to 1.5 μg lupeol and the correlation coefficient, 0.999, was indicative of good linear dependence of peak area on concentration. The mean assay of lupeol was 2.902 ± 0.243 mg g −1 bark. The method enables reliable quantification of lupeol and good resolution and separation of lupeol from other constituents of Grewia tiliaefolia . To ascertain the purity of the peak from the test sample its in-situ reflectance spectrum was compared with that from standard lupeol; clear superimposibility indicated the purity of the peaks. Recovery values from 98.00 to 99.78% showed the reliability and reproducibility of the method were excellent. The HPTLC method proposed for quantitative monitoring of lupeol in Grewia tiliaefolia is rapid, simple, and accurate and can be used for routine quality testing.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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