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  • 1 Medical University of Gdańsk Department of Pharmacognosy Gen. J. Hallera 107 st. 80-416 Gdańsk Poland
  • | 2 M. Curie-Skłodowska University Department of Adsorption and Planar Chromatography, Faculty of Chemistry M. Curie-Skłodowska Sq. 3 20-031 Lublin Poland
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Conditions have been established for TLC analysis and determination of the flavonol truxinic esters daglesiosides I and II in species of the family Pinaceae . Optimization of the separation of eleven flavonoids, including daglesiosides I, II, III, and IV, trans -tiliroside, trans -ditiliroside, kaempferol, quercetin, isorhamnetin, hyperoside, and astragalin, which are present in the methanol extract of Pseudotsuga menziesii , was performed on two types of layer — glass plates coated with RP-18F 254S and diol F 254S . Good resolution was achieved on diol F 254S layers by use of multiple gradient development with a mixture of chloroform and ethyl acetate. Under these conditions the presence of daglesioside I was demonstrated in two other species of Pinaceae — Cedrus atlantica and Cedrus atlantica var. glauca — and the flavonoid complex occurring in Pseudotsuga menziesii was completely separated by use of 2D TLC on coupled silica gel and RP-18 layers.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
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Address
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Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
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Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
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Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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