JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 15: Issue 6
Chromatographic and spectroscopic analysis of the fluorescent compounds derived from monosaccharides on HPTLC-NH 2 plates
Authors:
G. Grygierczyk Silesian University Institute of Chemistry 9 Szkolna Str. 40-006 Katowice Poland

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Walter Fischer Merck KGaA Darmstadt Germany

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M. Sajewicz Silesian University Institute of Chemistry 9 Szkolna Str. 40-006 Katowice Poland

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P. Kuś Silesian University Institute of Chemistry 9 Szkolna Str. 40-006 Katowice Poland

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R. Wrzalik Silesian University Institute of Physics 4 Uniwersytecka Str. 40-006 Katowice Poland

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M. Czaja Silesian University Faculty of Earth Sciences 60 Będzińska Str 41-200 Sosnowiec Poland

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M. Dziadek Silesian University Institute of Chemistry 9 Szkolna Str. 40-006 Katowice Poland

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Teresa Kowalska Silesian University Institute of Chemistry 9 Szkolna Str. 40-006 Katowice Poland

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Pages:
449–453
Online Publication Date:
13 Mar 2007
Publication Date:
01 Dec 2002
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.15.2002.6.11
Keywords:
3-Aminopropyl chemically bonded stationary phases; Monosaccharides; Maillard reaction; Fluorescence
Restricted access

In this paper we present results from recent studies focusing on elucidation of the mechanism of visualization of simple sugars (e.g. d -(+)-glucose, d -(+)-galactose, and d -(−)-fructose) developed on glass TLC plates precoated with 3-aminopropyl chemically bonded stationary phase and then heated at elevated temperatures, a method originally developed, then recommended commercially, by Merck.Detection of the sugars under UV illumination is possible because of their substantial fluorescence; this suggests that during heating the analytes probably undergo a process which results in their structural transformation. We postulated a possible analogy with the Maillard reaction, omnipresent in innumerable living organisms.To verify our assumption we performed analysis with high-performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), and IR, UV, and fluorescence spectroscopy. All the results obtained seem to confirm the analogy between the Maillard reaction and the reaction of simple carbohydrates after development on the amino stationary phase.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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