View More View Less
  • 1 L.M. College of Pharmacy Department of Quality Assurance Navrangpura, Ahmedabad 380 009 India
Restricted access

Cross Mark

Two fundamentally different analytical methods have been validated and used to monitor release of diclofenac and acetaminophen (paracetamol), from commercially available combination tablet formulations, in dissolution media. A sensitive HPTLC method was developed and validated for analysis of diclofenac. The filtered dissolution samples were directly spotted on to precoated silica gel 60 F 254 TLC plates and diclofenac was separated by use of chloroform-methanol-ammonia solution, 10 + 25 + 0.25 ( v/v ), as mobile phase. The spots were scanned at λ = 277 nm. Peak areas for diclofenac were found to be linearly dependent on the amount applied to the plate in the range 30–390 ng spot −1 ( r = 0.999).For estimation of acetaminophen a simple colorimetric method, involving reaction with sodium nitrite in acid then treatment with alkali to produce the 3-nitroacetaminophane anion (yellow color), was validated and used. The intensity of the yellow color, monitored at λ = 430 nm, was found to be linearly dependent on acetaminophen concentration in the range 5–30 μg mL −1 ( r = 0.999). These two methods were successfully used to study the dissolution profiles of diclofenac and acetaminophen from samples of ten different commercial diclofenac-acetaminophen combination tablets.

  • P.A. Insel in J.G. Hardman and L.E. Limbird (Eds) Goodman and Gilman’s The Pharmacological Basis of Therapeutics, 9th edn, McGraw-Hill, NY, 1996, pp. 617–657.

    Insel P.A. , '', in Goodman and Gilman’s The Pharmacological Basis of Therapeutics , (1996 ) -.

  • USP DI, 13th edn, United States Pharmacopeial Convention, Rockville, MD, 1993, pp. 1–7.

  • K. Zheng, Y. Chen , and H. Xie , CN 1,098,623 (Cl. A61K31/19) 15 Feb 1995.

  • The Indian Pharmacopoeia, Vol. II, Government of India, Ministry of Health and Family Welfare, The Controller of Publications, New Delhi, 1996, pp. 242–244.

  • The Indian Pharmacopoeia, Government of India, Ministry of Health and Family Welfare, The Controller of Publications, New Delhi, 1996, Addendum (2000), p. 874.

  • British Pharmacopoeia, Vol. II, HMSO, Cambridge, International edn, 1999, pp. 2016–2018.

  • British Pharmacopoeia, Vol. II, HMSO, Cambridge, International edn, 1999, pp. 1779–1781.

  • USP 24, NF 19, United States Pharmacopeial Convention, Rockville, MD, Asian edn, 2000, pp. 546–547.

  • USP 24, NF 19, United States Pharmacopeial Convention, Rockville, MD, Asian edn, 2000, Supplement 1, p. 2591.

  • H.L. Rau, A.R. Aroor , and P. Gundurao , Indian Drugs 28 (1991) 285–286.

    Gundurao P. , '' (1991 ) 28 Indian Drugs : 285 -286.

  • K. Lahiri, G. K. Babu , and R.S.R. Murthy , The Eastern Pharmacist 35 (1992) 117–119.

    Murthy R.S.R. , '' (1992 ) 35 The Eastern Pharmacist : 117 -119.

  • A.P. Argekar and S.J. Shah , Indian Drugs 34 (1997) 437–442.

    Shah S.J. , '' (1997 ) 34 Indian Drugs : 437 -442.

  • P.D. Sethi (Ed.) Quantitative Analysis of Drugs in Pharmaceutical Formulations, 2nd edn, CBS, New Delhi, 1993, p. 93.

    '', in Quantitative Analysis of Drugs in Pharmaceutical Formulations , (1993 ) -.

  • M.S. Bhatia, S.G. Kaskhedikar , and S.C. Chaturvedi , Indian Drugs 34 (1997) 149–153.

    Chaturvedi S.C. , '' (1997 ) 34 Indian Drugs : 149 -153.

  • M.S. Bhatia and S.R. Dhaneshwar , Indian Drugs 32 (1995) 446–450.

    Dhaneshwar S.R. , '' (1995 ) 32 Indian Drugs : 446 -450.

  • R.T. Sane, R.S. Samant , and V.G. Nayak , Indian Drugs 24 (1986) 161–162.

    Nayak V.G. , '' (1986 ) 24 Indian Drugs : 161 -162.

  • Y.K. Agrawal, V.P. Upadhyay , and S.K. Menon , Indian J. Pharm. Sci. 50 (1988) 58–60.

    Menon S.K. , '' (1988 ) 50 Indian J. Pharm. Sci. : 58 -60.

  • P.D. Sethi (Ed.) HPTLC: Quantitative Analysis of Pharmaceutical Formulations, 1st edn, CBS, New Delhi, 1996, pp. 162–165.

    '', in HPTLC: Quantitative Analysis of Pharmaceutical Formulations , (1996 ) -.

  • V.M. Shinde, N.M. Tendolkar , and B.S. Desai , J. Planar. Chromatogr. 7 (1994) 50–53.

    Desai B.S. , '' (1994 ) 7 J. Planar. Chromatogr. : 50 -53.

  • P.D. Sethi (Ed.), HPTLC: Quantitative Analysis of Pharmaceutical Formulations, 1st edn, CBS, New Delhi, 1996, pp. 188–189.

    '', in HPTLC: Quantitative Analysis of Pharmaceutical Formulations , (1996 ) -.

  • J.W. Murfin , Analyst 97 (1972) 663–669.

    Murfin J.W. , '' (1972 ) 97 Analyst : 663 -669.

  • R.D. Deodhar, M.R. Shastri , and R.C. Mehta , Indian J. Pharm. 38 (1976) 18–21.

    Mehta R.C. , '' (1976 ) 38 Indian J. Pharm. : 18 -21.

  • F. Le Perdriel, C. Hanegraaff, N. Chastagner , and E. Demontety , Ann. Pharm. Fr. 26 (1968) 227–237.

    Demontety E. , '' (1968 ) 26 Ann. Pharm. Fr. : 227 -237.

  • USP 24, NF 19, United States Pharmacopeial Convention, Rockville, MD, Asian edn., 2000, pp. 20–40.

  • J.E. Fairbrother in K. Florey (Ed.) Analytical Profiles of Drug Substances, Vol. 3, Academic Press, NY, 1974, pp. 1–111.

    Fairbrother J.E. , '', in Analytical Profiles of Drug Substances, Vol. 3 , (1974 ) -.

  • W. Lund (Ed.) The Pharmaceutical Codex — Principles and Practice of Pharmaceutics, 12th edn, Pharmaceutical Press, London, 1994, 835–838.

    '', in The Pharmaceutical Codex — Principles and Practice of Pharmaceutics , (1994 ) -.

All Time Past Year Past 30 Days
Abstract Views 38 38 2
Full Text Views 9 6 0
PDF Downloads 7 3 0