JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 16: Issue 2
New HPTLC method for identification and quantification of folic acid from pharmaceutical dosage preparations
Authors:
V. Dongre Dr Babasaheb Ambedkar Marathwada University Department of Chemistry Aurangabad 431004 India

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V. Bagul Dr Babasaheb Ambedkar Marathwada University Department of Chemistry Aurangabad 431004 India

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Pages:
112–116
Online Publication Date:
08 Mar 2007
Publication Date:
01 Apr 2003
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.16.2003.2.5
Keywords:
Folic acid; HPTLC; Pharmaceutical analysis
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A new HPTLC method has been developed for identification and quantification of folic acid, N -[4-{[(2-amino-4-hydroxy-6-pteridynyl)methyl]amine}benzoyl]glutamic acid, a water-soluble vitamin. Identification and quantification were studied with different mobile phases. The best results were obtained on HPTLC silica gel 60 F 254 plates (Merck) with iso -propanol-ethyl acetate-water-ammonia, 4 + 2 + 2 + 1, as mobile phase. Quantification was achieved by use of densitometry at λ = 280 nm, using the deuterium lamp. Recovery, linearity, reproducibility, and accuracy were excellent for all the samples studied. The method has been applied successfully for the identification and quantification of folic acid in different pharmaceutical dosage preparations. The standard and assay preparations are stable for up to 48 h.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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