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  • 1 Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy 6 Chodźki Str. 20-093 Lublin Poland
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A new, simple, and accurate TLC method, using normal- and reversed-phase techniques and densitometric detection, has been developed for measurement of quinapril and hydrochlorothiazide in combination tablets. UV detection at λ = 210 nm was used to quantify the analytes. The drugs were chromatographed on silica gel 60 F 254 HPTLC plates and on octadecilsilane (RP-18) TLC plates, in horizontal chambers, with ethyl acetate-acetone-acetic acid, 8 + 2 + 0.5 ( v/v ) and methanol-0.07 m phosphate buffer, pH 2.5, 6 + 4 ( v/v ), respectively, as mobile phases. The active substances were extracted from tablets with methanol (96% < mean recovery < 104%). Calibration curves were constructed in the range 0.4 to 2.4 μg μL −1 for quinapril and 0.25 to 1.5 μg μL −1 for hydrochlorothiazide, with good correlation ( r ≥ 0.998). The precision ( RSD < 4.4%) and accuracy (2.91 < RE < 3.92) were satisfactory for TLC-densitometric determination of quinapril in combination with hydrochlorothiazide in commercial tablets.

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