A sensitive high-performance thin-layer chromatographic (HPTLC) method has been developed for estimation of amentoflavone, an important bioactive biflavonoid found in many medicinal plants. Amentoflavone was isolated from
, characterized, and used as reference standard. The HPTLC method was validated for precision (intra- and inter-day), repeatability, and accuracy. The method was found to be precise — intra-day and inter-day
, for different concentrations, were 0.52–1.36% and 1.87–3.41%, respectively. Instrumental precision and repeatability of the method were found to be 0.098 and 2.1 (%
), respectively. The accuracy of the method was checked by performing a recovery study at two levels; average recovery was found to be 98.86%. The method was used for the estimation of amentoflavone from
. The method was found to be accurate and precise and could be used for the estimation of amentoflavone from plant materials.
, Trease and Evans’ Pharmacognosy, 14th edn, Harcourt Brace Asia, 1996, pp. 249–252.
S.S. Gambhir, R.K. Goel
, Indian J. Med. Res.
Dasgupta G. , '' (1987) 85Indian J. Med. Res.: 689-693.
Dasgupta G. Indian J. Med. Res.198785689693)| false
F. Bucar, S.M. Jachak, T. Kartnig
Schubert-Zsilavecz M. , '' (1998) 53Pharmazie: 651-652.
Schubert-Zsilavecz M. Pharmazie199853651652)| false
F. Bucar, S.M. Jackak, Y. Noreen, T. Kartning, P. Perera, L. Bohlin
, Planta Med.
Schubert-Zsilavecz M. , '' (1998) 64Planta Med.: 373-374.
Schubert-Zsilavecz M. Planta Med.199864373374)| false
H.K. Kim, K.H. Son, H.W. Chang, S.S. Kang
, Arch. Pharm. Res.
Kim H.P. , '' (1998) 21Arch. Pharm. Res.: 406-410.
Kim H.P. Arch. Pharm. Res.199821406410)| false
H.P. Kim, I. Mani, L. Iversen
, Leukot. Essent. Fatty Acids