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  • 1 B.V. Patel Pharmaceutical Education and Research Development Center Thaltej, Ahmedabad 380054 India
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A sensitive high-performance thin-layer chromatographic (HPTLC) method has been developed for estimation of amentoflavone, an important bioactive biflavonoid found in many medicinal plants. Amentoflavone was isolated from Biophytum sensitivum , characterized, and used as reference standard. The HPTLC method was validated for precision (intra- and inter-day), repeatability, and accuracy. The method was found to be precise — intra-day and inter-day RSD , for different concentrations, were 0.52–1.36% and 1.87–3.41%, respectively. Instrumental precision and repeatability of the method were found to be 0.098 and 2.1 (% CV ), respectively. The accuracy of the method was checked by performing a recovery study at two levels; average recovery was found to be 98.86%. The method was used for the estimation of amentoflavone from B. sensitivum and Putranjiva roxburghii . The method was found to be accurate and precise and could be used for the estimation of amentoflavone from plant materials.

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