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  • 1 University of Belgrade Department of Pharmacokinetics, Faculty of Pharmacy P.O. Box 146 Belgrade Serbia and Montenegro
  • 2 University of Belgrade Department of Pharmaceutical Chemistry, Faculty of Pharmacy P.O. Box 146 Belgrade Serbia and Montenegro
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A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method time-saving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction — recoveries were 99 ± 8% (mean ± SD ). Chromatography was performed on silica gel 60 F 254 TLC plates, developed with toluene-acetone, 100 + 10 ( v/v ) and evaluated densitometrically at λ = 310 nm in reflectance-absorbance mode. The RF was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L −1 ) and within-run and day-to-day coefficients of variation of 2.8–4.5% and 4.8–8.5%, respectively. The method was used to determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration.

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