Author:
Gertrud Morlock University of Hohenheim Institute of Food Chemistry D-70599 Stuttgart Germany

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A new HPTLC method has been established for separation and quantification of six given apolar, fluorescent pyrolytic heterocyclic aromatic amines (HA) of the carboline group. The mobile phase was selected by using a practical and systematic four-level optimization scheme based on the solvent classification system according to Snyder and the PRISMA model of Nyiredy et al. The amines were separated on silica gel 60 F 254 HPTLC plates by a multiple development with diethyl ether. Quantification by fluorescence measurement at λ = 366/>400 nm was performed immediately after development. Limits of detection were in the lownanogram range. The results show that modern planar chromatography is a rapid and cost-efficient alternative method for determination of HA. The method is suitable for both screening and quantification.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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