View More View Less
  • 1 Medical University of Gdańsk Department of Pharmacognosy al. Gen. J. Hallera 107 80-416 Gdańsk Poland
Restricted access

Conditions have been established for TLC separation of a mixture of nine quinolizidine alkaloid standards (lupanine, methylcytisine, cytisine, hydroxylupanine, sophocarpine, lusitanine, retamine, sparteine, and l - α -isosparteine) and of alkaloid fractions from a variety of Genista species and from in-vitro cultures of members of this species. Optimization of the one-dimensional TLC separation of the nine alkaloid standards was performed on two types of layer, RP-18W and silica gel. The mixture of standards was also chromatographed by two-dimensional TLC on diol F 254S layers and by 2D TLC with an adsorbent gradient. In the last variant (so-called graft TLC) the first development was performed on a TLC diol plate and the second, in the perpendicular direction, on an RP-18W TLC plate. Only by 2D TLC with an adsorbent gradient was it possible to achieve full separation both of the standards and of alkaloid fractions from plant material. Under these conditions the presence of retamine, cytisine, sparteine, l - α -isosparteine, lupanine, and methylcytisine was discovered in all Genista species and in shoot cultures of G. tinctoria .

  • A.D. Kinghorn and M.F. Balandrin , in: S.W. Pelletier (Ed.) Alkaloids: Chemical and Biological Perspectives, Vol. 2, Wiley, New York, 1984, p. 105.

    Balandrin M.F. , '', in Alkaloids: Chemical and Biological Perspectives, Vol. 2 , (1984 ) -.

  • S. Ohmiya, K. Saito , and I. Murakoshi , In: G.A. Cordell (Ed.) The alkaloids, Vol. 47, Academic Press, New York, 1995, pp. 1–114.

    Murakoshi I. , '', in The alkaloids, Vol. 47 , (1995 ) -.

  • M. Wink and T. Hartmann , Planta Med. 40 (1980) 31–39.

    Hartmann T. , '' (1980 ) 40 Planta Med. : 31 -39.

  • M. Wink, L. Witte , and T. Hartmann , Planta Med. 43 (1981) 342–352.

    Hartmann T. , '' (1981 ) 43 Planta Med. : 342 -352.

  • M. Wink , Planta Med. 74 (1987) 509–513.

    Wink M. , '' (1987 ) 74 Planta Med. : 509 -513.

  • W. Cisowski, W. Dembińska-Migas, M. Krauze-Baranowska, M. Łuczkiewicz, P. Migas, G. Matysik , and E. Soczewiński , J. Planar Chromatogr. 11 (1998) 441–446.

    Soczewiński E. , '' (1998 ) 11 J. Planar Chromatogr. : 441 -446.

  • M. Wink, L. Witte, H.M. Schiebel , and T. Hartmann , Planta Med. 38 (1980) 238–245.

    Hartmann T. , '' (1980 ) 38 Planta Med. : 238 -245.

  • S. Jing-Zheng, X. Hong-Xi, T. Song-Jiu , and P. Pui-Hay But , J. Chromatogr. A 857 (1999) 303–311.

    Pui-Hay But P. , '' (1999 ) 857 J. Chromatogr. A : 303 -311.

  • T. Ebner, C.O. Meese , and M. Eichelbaum , Ther. Drug Monit. 11 (1989) 214.

    Eichelbaum M. , '' (1989 ) 11 Ther. Drug Monit. : 214 -.

  • L.X. Jin, Y.Y. Cui , and G.D. Zhang , Acta Pharm. Sinica 28 (1993) 136.

    Zhang G.D. , '' (1993 ) 28 Acta Pharm. Sinica : 136 -.

  • F. Tosun, M. Tanker, T. Özden , and A. Tosun , Planta Medica 34 (1986) 242.

    Tosun A. , '' (1986 ) 34 Planta Medica : 242 -.

  • M. Gleńsk, Z. Biały, M. Jurzysta , and W. Cisowski , Chromatographia 54 (2001) 669–670.

    Cisowski W. , '' (2001 ) 54 Chromatographia : 669 -670.

  • M. Gleńsk, U. Sawicka, I. Mażol , and W. Cisowski , J. Planar Chromatogr. 15 (2002) 463–465.

    Cisowski W. , '' (2002 ) 15 J. Planar Chromatogr. : 463 -465.

  • Sz. Nyiredy , Multidimensional Planar Chromatography, In: Sz. Nyiredy (Ed.) Planar Chromatography — A Retrospective View for the Third Millennium, Springer, Hungary, 2001, pp. 107–109.

    Nyiredy Sz. , '', in Planar Chromatography — A Retrospective View for the Third Millennium , (2001 ) -.

  • A. Hermans-Lokkerbol and R. Verpoorte , Planta Med. 41 (1986) 299–302.

    Verpoorte R. , '' (1986 ) 41 Planta Med. : 299 -302.

All Time Past Year Past 30 Days
Abstract Views 53 53 2
Full Text Views 15 9 1
PDF Downloads 21 17 2