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  • 1 University of Applied Sciences Offenburg Badstrasse 24 77652 Offenburg Germany
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A new formula is presented for transforming fluorescence measurements in accordance with Kubelka-Munk theory. The fluorescence signals, the absorption signals, and data from a selected reference are combined in one expression. Only diode-array techniques can measure all the required data simultaneously to linearize fluorescence data correctly. To prove the new theory HPTLC quantification of the analgesic flupirtine was performed over the mass range 300 to 5000 ng per spot. The fluorescence calibration curve was linear over the whole range. The transformation of fluorescence measurements into linear mass-dependent data extends the technique of in-situ fluorescence analysis to the high concentration range. It also extends Kubelka-Munk theory from absorption to fluorescence analysis. The results presented also emphasize the importance of Kubelka-Munk theory for in-situ measurements in scattering media, especially in planar chromatography.

  • J. Sherma and B. Fried (Eds) Handbook of Thin-Layer Chromatography, 3rd edn, Marcel Dekker, New York, 2003.

    '', in Handbook of Thin-Layer Chromatography , (2003 ) -.

  • J.C. Touchstone and J. Sherma , in: Densitometry in Thin-Layer Chromatography, John Wiley and Sons New York, 1979.

    Sherma J. , '', in Densitometry in Thin-Layer Chromatography , (1979 ) -.

  • S. Ebel , Topics Curr. Chem. 126 (1984) 71.

    Ebel S. , '' (1984 ) 126 Topics Curr. Chem. : 71 -.

  • B. Spangenberg and K.-F. Klein , J. Chromatogr. A 898 (2000) 265–269.

    Klein K.-F. , '' (2000 ) 898 J. Chromatogr. A : 265 -269.

  • P. Kubelka and F. Munk , Z. Tech. Physik 11a (1931) 593–601.

    Munk F. , '' (1931 ) 11a Z. Tech. Physik : 593 -601.

  • P. Kubelka and F. Munk , J. Opt. Soc. Am. 38 (1948) 448, 1067.

    Munk F. , '' (1948 ) 38 J. Opt. Soc. Am. : 448 -.

  • G. Kortüm , Reflextionsspektroskopie, Springer, 1969.

  • D. Oelkrug and G. Kortüm , Z. Physik. Chemie 56 (1968) 181–188.

    Kortüm G. , '' (1968 ) 56 Z. Physik. Chemie : 181 -188.

  • P. Post , Massenverteilung und densitometrische Detektion in der DC, thesis, Marburg, 1979.

  • B. Spangenberg, P. Post , and S. Ebel , J. Planar Chromatogr. 15 (2002) 11–18.

    Ebel S. , '' (2002 ) 15 J. Planar Chromatogr. : 11 -18.

  • B. Ahrens, D. Blankenhorn , and B. Spangenberg , J. Chromatogr. B 772 (2002) 11–18.

    Spangenberg B. , '' (2002 ) 772 J. Chromatogr. B : 11 -18.

  • B. Spangenberg, B. Ahrens , and K.-F. Klein , Chromatographia 53 (2001) 438–441.

    Klein K.-F. , '' (2001 ) 53 Chromatographia : 438 -441.

  • B. Spangenberg and K.-F. Klein , J. Planar Chromatogr. 14 (2001) 260–265.

    Klein K.-F. , '' (2001 ) 14 J. Planar Chromatogr. : 260 -265.

  • J. Stroka, B. Spangenberg , and E. Anklam , J. Liq. Chromatogr. Related Technol. 25 (2002) 1497–1513.

    Anklam E. , '' (2002 ) 25 J. Liq. Chromatogr. Related Technol. : 1497 -1513.

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