Authors:
Tomás López Animal Toxicology Laboratory Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria (INTA) C. C. 276, Balcarce (7620) Buenos Aires Argentina

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Mónica Torre Universidad Nacional de Mar del Plata (FCA; UNMdP) Facultad de Ciencias Agrarias C. C. 276, Balcarce (7620) Buenos Aires Argentina

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María Cid UNMdP and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) FCA Argentina

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There is no information about the risk to Argentinean livestock of poisoning by exposure to Conium maculatum L. as a result of ingestion of its two most toxic alkaloids, γ -coniceine and coniine. To measure their concentrations in this weed an aqueous sulfuric acid extract of C. maculatum foliage alkaloids was adjusted to pH 9 and extracted exhaustively with chloroform. The partly evaporated chloroform extract was used for TLC detection and quantification of γ -coniceine and coniine by using Dragendorff’s spray reagent to detect the alkaloids and visual comparison of the intensity of the color of the sample spots with that of the spots of the corresponding standards. Regression equations for recoveries ( y ) after different standard additions ( x ) were y = −0.761 + 1.051 x ( r2 = 0.93; p < 0.0001) for coniine and y = −0.193 + 0.863 x ( r2 = 0.93; p < 0.0001) for γ -coniceine. Average recoveries were 92.0 ± 3.5% and 82.6 ± 3.9% for coniine and γ -coniceine, respectively. By t -tests on addition and recovery values it was shown that for both equations α = 0 and β = 1 ( p > 0.05), which implies recovery is 100%. Detection limits were 1.7 and 0.7 μg per spot for coniine and γ -coniceine, respectively; the respective quantification limits were 0.8 and 0.6 μg mL −1 . The method is suitable for simple, precise, and accurate TLC analysis of these alkaloids.

  • Aristocle (Plato) , in Phaedo, Dialog 66, Athens (ca. 380BC).

  • T.A. López, M.S. Cid , and M.R. Bianchini , Toxicon 37 (1999) 841–865.

    Bianchini M.R. , '' (1999 ) 37 Toxicon : 841 -865 .

  • R.F. Keeler , in: R.F. Keeler, K. van Kampen , and L.F. James (Eds) Effects of Poisonous Plants on Livestock, Academic Press, New York, 1987, pp. 401–404.

    Keeler R.F. , '', in Effects of Poisonous Plants on Livestock , (1987 ) -.

  • L.D. Edmons, L.A. Selby , and A.A. Case , J. Am. Vet. Med. Assoc. 160 (1972) 1319–1324.

    Case A.A. , '' (1972 ) 160 J. Am. Vet. Med. Assoc. : 1319 -1324 .

  • K.E. Panter and R.F. Keeler , in: P.R. Cheeke (Ed.), Toxicants of Plant Origin, CRC Press, Boca Raton, 1989, p. 113.

    Keeler R.F. , '', in Toxicants of Plant Origin , (1989 ) -.

  • M.L. de la Torre, T.A. López, M.S. Cid, M.A. Brizuela , and J. García , Rev. Arg. Prod. Anim. 17 (Suppl. 1) (1997) 323–324.

    García J. , '' (1997 ) 17 Rev. Arg. Prod. Anim. : 323 -324 .

  • IARC Monographs on the Evaluation of Carcinogenic Risks to Humans Suppl. 7, IARC Press, Geneva, 1987, 152–154.

  • K.G. Krebs, D. Heusser , and H. Wimmer , in: E. Stahl (Ed.) Thin-Layer Chromatography, Springer, Berlin, 1969, p. 874.

    Wimmer H. , '', in Thin-Layer Chromatography , (1969 ) -.

  • The Merck Index, 12th edn, Merck and Co., Rahway, 1996, pp. 423–424.

  • Levene (Med) Test. In: R.O. Kuehl (Ed.) Statistical Principles of Research Design and Analysis, Duxbury Press, 1994, pp. 113–116.

  • M.L. de la Torre, T.A. López , and M.S. Cid , Proc. 16th Latin American Meeting on Animal Production, Montevideo, 2000, on CD.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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