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  • 1 Animal Toxicology Laboratory Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria (INTA) C. C. 276, Balcarce (7620) Buenos Aires Argentina
  • 2 Universidad Nacional de Mar del Plata (FCA; UNMdP) Facultad de Ciencias Agrarias C. C. 276, Balcarce (7620) Buenos Aires Argentina
  • 3 UNMdP and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) FCA Argentina
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There is no information about the risk to Argentinean livestock of poisoning by exposure to Conium maculatum L. as a result of ingestion of its two most toxic alkaloids, γ -coniceine and coniine. To measure their concentrations in this weed an aqueous sulfuric acid extract of C. maculatum foliage alkaloids was adjusted to pH 9 and extracted exhaustively with chloroform. The partly evaporated chloroform extract was used for TLC detection and quantification of γ -coniceine and coniine by using Dragendorff’s spray reagent to detect the alkaloids and visual comparison of the intensity of the color of the sample spots with that of the spots of the corresponding standards. Regression equations for recoveries ( y ) after different standard additions ( x ) were y = −0.761 + 1.051 x ( r2 = 0.93; p < 0.0001) for coniine and y = −0.193 + 0.863 x ( r2 = 0.93; p < 0.0001) for γ -coniceine. Average recoveries were 92.0 ± 3.5% and 82.6 ± 3.9% for coniine and γ -coniceine, respectively. By t -tests on addition and recovery values it was shown that for both equations α = 0 and β = 1 ( p > 0.05), which implies recovery is 100%. Detection limits were 1.7 and 0.7 μg per spot for coniine and γ -coniceine, respectively; the respective quantification limits were 0.8 and 0.6 μg mL −1 . The method is suitable for simple, precise, and accurate TLC analysis of these alkaloids.

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