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  • 1 Silesian University of Technology Department of Analytical and General Chemistry 7 M. Strzody Str. 44-100 Gliwice Poland
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Relationships between RF values and mobile-phase composition have been determined for six methylxanthines and acidic and neutral biogenic amine metabolites on silica gel with mobile phases comprising a non-polar or weakly polar diluent (chloroform, heptane, or hexane) and a polar modifier (THF, dioxane, acetone, or ethyl acetate). The relationships obtained constitute a retention database providing useful information for TLC sample-screening methods and for isolation and preconcentration of the compounds of interest by solid-phase extraction (SPE) on silica columns.

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