JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 17: Issue 4
Analysis of glycosides and aglycones of flavonoid compounds by double-development thin-layer chromatography
Authors:
E. Soczewiński Medical University Department of Chemistry Staszica 6 20-081 Lublin Poland

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M. Wójciak-Kosior Medical University Department of Chemistry Staszica 6 20-081 Lublin Poland

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G. Matysik Medical University Department of Chemistry Staszica 6 20-081 Lublin Poland

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Pages:
261–263
Online Publication Date:
08 Mar 2007
Publication Date:
01 Aug 2004
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.17.2004.4.4
Keywords:
Thin-layer chromatography; Flavonoid glycosides and aglycones; Incremental multiple development; Two-step development
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Flavonoid glycosides are much more polar than their aglycones and the two groups of compounds are difficult to separate by planar chromatography owing to the ‘general elution problem’ — glycosides require development with mobile phases of higher eluent strength. The problem of separating both groups can be solved by gradient elution or by incremental gradient multiple development in its simplest form — double development. The latter technique is illustrated for nine glycosides and seven aglycones by development along two-thirds of the plate length with a strong mobile phase and, after evaporation of the solvent, development to the full distance with a weaker mobile phase. The two stages of development are illustrated by densitograms.

  • G. Matysik and E. Soczewiński , J. Planar Chromatogr. 9 (1996) 404–412; Fig.6.

    Soczewiński E. , '' (1996 ) 9 J. Planar Chromatogr. : 404 -412 .

  • A. Jeanes, C.S. Wise , and R.J. Dimler , Anal. Chem. 23 (1951) 415–419.

    Dimler R.J. , '' (1951 ) 23 Anal. Chem. : 415 -419 .

  • C.C. Woodward and G.S. Racideau , Anal. Chem. 26 (1954) 248–254.

    Racideau G.S. , '' (1954 ) 26 Anal. Chem. : 248 -254 .

  • U. de la Vigne and D. Jänchen , J. Planar Chromatogr. 3 (1990) 6–11.

    Jänchen D. , '' (1990 ) 3 J. Planar Chromatogr. : 6 -11 .

  • G. Lodi, A. Botti, E. Mensiani, V. Brandolini , and B. Tosi , J. Planar Chromatogr. 4 (1991) 106–112.

    Tosi B. , '' (1991 ) 4 J. Planar Chromatogr. : 106 -112 .

  • C.F. Poole, S.K. Poole, W.P.N. Fernando, T.A. Dean, H.D. Ahmed , and J.A. Berndt , J. Planar Chromatogr. 2 (1989) 336–341.

    Berndt J.A. , '' (1989 ) 2 J. Planar Chromatogr. : 336 -341 .

  • N. Grinberg (Ed.) Modern Thin-Layer Chromatography, Marcel Dekker, New York, 1990, p. 292.

    '', in Modern Thin-Layer Chromatography , (1990 ) -.

  • K. Burger , Fresenius Z. Anal. Chem. 23 (1951) 415–419.

    Burger K. , '' (1951 ) 23 Fresenius Z. Anal. Chem. : 415 -419 .

  • C.F. Poole , J. Chromatogr. A 856 (1999) 399–345.

    Poole C.F. , '' (1999 ) 856 J. Chromatogr. A : 399 -345 .

  • Sz. Nyiredy (Ed.) Planar Chromatography. A Retrospective View for the Third Millennium, Springer, Budapest, 2001.

    '', in Planar Chromatography. A Retrospective View for the Third Millennium , (2001 ) -.

  • F. Geiss , Fundamentals of Thin-Layer Chromatography (Planar Chromatography), Hüthig, Heidelberg, 1987, p. 144.

    Geiss F. , '', in Fundamentals of Thin-Layer Chromatography (Planar Chromatography) , (1987 ) -.

  • L.-A. Johansson , Sver. Utsådesforen. Tidskr. 95 (1985) 129–137.

    Johansson L.-A. , '' (1985 ) 95 Sver. Utsådesforen. Tidskr. : 129 -137 .

  • W. Markowski and E. Soczewiński , J. Chromatogr. 623 (1992) 139–147.

    Soczewiński E. , '' (1992 ) 623 J. Chromatogr. : 139 -147 .

  • W. Markowski , J. Chromatogr. 635 (1993) 283–288.

    Markowski W. , '' (1993 ) 635 J. Chromatogr. : 283 -288 .

  • W. Markowski and E. Soczewiński , Chromatographia 36 (1993) 330–336.

    Soczewiński E. , '' (1993 ) 36 Chromatographia : 330 -336 .

  • W. Markowski, K. Czapińska , and T. Baj , J. Planar Chromatogr. 16 (2003) 214–219.

    Baj T. , '' (2003 ) 16 J. Planar Chromatogr. : 214 -219 .

  • T.H. Dzido and E. Soczewiński , J. Chromatogr. 516 (1990) 461–466; www.chromdes.com.

    Soczewiński E. , '' (1990 ) 516 J. Chromatogr. : 461 -466 .

  • T.H. Dzido , Modern TLC Chambers. In: Sz. Nyiredy (Ed.) Planar Chromatography. A Retrospective View for the Third Millennium, Springer, Budapest, 2001, Chapter 4, pp. 68–87.

    Dzido T.H. , '', in Planar Chromatography. A Retrospective View for the Third Millennium , (2001 ) -.

  • L.R. Snyder, J.J. Kirkland , and J.L. Glajch , Practical HPLC Method Development, 2nd edn, Wiley-Interscience, New York, 1997, pp. 353ff.

    Glajch J.L. , '', in Practical HPLC Method Development , (1997 ) -.
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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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