JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 17: Issue 4
Thin-layer chromatographic analysis of flavonoids, phenolic acids, and amino acids in some Croatian Hypericum taxa
Authors:
Željan Maleš University of Zagreb Department of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry Schrottova 39 10000 Zagreb Croatia

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Miško Plazibat University of Zagreb Department of Botany, Faculty of Science Marulićev trg 20/II 10000 Zagreb Croatia

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Vjera Vundać University of Zagreb Department of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry Schrottova 39 10000 Zagreb Croatia

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Irena Žuntar University of Zagreb Faculty of Pharmacy and Biochemistry A. Kovačića 1 10000 Zagreb, Croatia USA

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Kroata Pilepić University of Zagreb Department of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry Schrottova 39 10000 Zagreb Croatia

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Pages:
280–285
Online Publication Date:
08 Mar 2007
Publication Date:
01 Aug 2004
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.17.2004.4.7
Restricted access

The flowering tops of Hypericum hirsutum, H. montanum, H. perforatum subsp. angustifolium, H. perforatum subsp. perforatum , and H. perforatum subsp. veronense , collected from different locations in Croatia, were investigated to determine their flavonoid, phenolic acid, and amino acid composition. Thin-layer chromatography (TLC) on silica gel was used for separation and identification of the flavonoids and phenolic acids and TLC on cellulose was used for investigation of the amino acids. By use of these procedures ten flavonoids, three phenolic acids, and sixteen amino acids were detected and identified in the samples investigated. The composition of these compounds depended on the taxa investigated and the locality. Spectrophotometric analysis of the flavonoid content indicated that the three subspecies of H. perforatum are richer in flavonoids than H. hirsutum and H. montanum. H. perforatum subsp. perforatum is the richest in flavonoids, phenolic acids, and amino acids. This is the first time these biologically active compounds have been identified in the some Hypericum taxa.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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