JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 17: Issue 4
Characterization and TLC-bioautographic detection of essential oils from some Thymus taxa. Determination of the activity of the oils and their components against plant pathogenic bacteria
Authors:
Györgyi Horváth University of Pécs Department of Botany, Faculty of Natural Sciences Ifjúság útja 6 H-7624 Pécs Hungary

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László Szabó University of Pécs Department of Botany, Faculty of Natural Sciences Ifjúság útja 6 H-7624 Pécs Hungary

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Éva Lemberkovics Semmelweis University Department of Pharmacognosy, Faculty of Pharmacy Üllői út 26 H-1085 Budapest Hungary

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Lajos Botz University of Pécs Pharmaceutical Institute, Medical School Honvéd u. 3 H-7624 Pécs Hungary

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Béla. Kocsis University of Pécs Department of Medical Microbiology and Immunology, Medical School Szigeti u. 12 H-7624 Pécs Hungary

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Pages:
300–304
Online Publication Date:
08 Mar 2007
Publication Date:
01 Aug 2004
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.17.2004.4.11
Keywords:
Thin layer chromatography, TLC; Gas chromatography, GC; Direct bioautography; Essential oil; Plant pathogenic bacteria; Thymus taxa
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The chemical composition of the oils of four thyme (Lamiaceae) chemotypes ( Thymus vulgaris L., Thymus serpyllum L., Thymus x citriodorus (Pers.) Schreb., and Thymus x citriodorus “Archer’s Gold”) has been determined by gas chromatography (GC). Thymol was the main component of the oils of Thymus vulgaris and Thymus serpyllum , geraniol was the main component of the oil of Thymus x citriodorus , and carvacrol was the main component of the oil of Thymus x citriodorus “Archer’s Gold”. The bioactivity of the volatile oil of Thymus vulgaris and of the three main components of the oils against Gram negative plant pathogenic bacteria was examined by direct bioautography. They had an inhibitory effect on all of the test microorganisms. Two bacterial strains ( Xanthomonas campestris pv. vesicatoria and Pseudomonas syringae pv. phaseolicola ) were most sensitive in the bioautographic system and use of these bacteria would considerably shorten the process of bioautographic detection. The two antibiotics gentamycin and streptomycin were used as controls.

  • B.M. Lawrence , Perfum. Flavor. 15 (5) (1990) 59.

    Lawrence B.M. , '' (1990 ) 15 Perfum. Flavor. : 59 -.

  • E.J. Stahl-Biskup , J. Essent. Oil Res. 3 (1991) 61.

    Stahl-Biskup E.J. , '' (1991 ) 3 J. Essent. Oil Res. : 61 -.

  • H.J. Storesund, N. Asfaw, L. Skattebol, F. Tonnesen , and A. J. Aasen , Flavour Frag. J. 15 (2000) 123–125.

    Aasen A. J. , '' (2000 ) 15 Flavour Frag. J. : 123 -125 .

  • U. Asllani and V. Toska , J. Essent. Oil Res. 15 (2003) 165–167.

    Toska V. , '' (2003 ) 15 J. Essent. Oil Res. : 165 -167 .

  • D. Mockute and G. Bernotiene , Biochem. System. Ecol. 29 (2001) 69–76.

    Bernotiene G. , '' (2001 ) 29 Biochem. System. Ecol. : 69 -76 .

  • T. Adzet, S. Canigueral , and N. Gabalda , Phytochemistry 30 (7) (1991) 2289–2293.

    Gabalda N. , '' (1991 ) 30 Phytochemistry : 2289 -2293 .

  • D. Biondi, P. Cianci, C. Geraci, G. Ruberto , and M. Piatelly , Flavour Frag. J. 8 (1993) 331–337.

    Piatelly M. , '' (1993 ) 8 Flavour Frag. J. : 331 -337 .

  • W.C. Evans , Evan’s Pharmacognosy, Bailliere Tindall, London, 1998.

    Evans W.C. , '', in Evan’s Pharmacognosy , (1998 ) -.

  • K.A. Hammer, C.F. Carson , and F.V. Riley , J. Appl. Microbiol. 86 (1999) 985–990.

    Riley F.V. , '' (1999 ) 86 J. Appl. Microbiol. : 985 -990 .

  • M. Marino, C. Bersani , and G. Comi , J. Food Prot. 62 (1999) 1017–1023.

    Comi G. , '' (1999 ) 62 J. Food Prot. : 1017 -1023 .

  • C. Juliano, A. Mattana , and M. Usai , J. Essent. Oil Res. 12 (2000) 516.

    Usai M. , '' (2000 ) 12 J. Essent. Oil Res. : 516 -.

  • S. Karaman, M. Digrak, U. Ravid , and A. Ilcim , J. Ethnopharmacol. 76 (2) (2001) 183.

    Ilcim A. , '' (2001 ) 76 J. Ethnopharmacol. : 183 -.

  • S. Cosentino, C.I.G. Tuberoso, B. Pisano, M. Satta, V. Mascia, E. Arzedi , and F. Palmas , Lett. Appl. Microbiol. 29 (1999) 130–135.

    Palmas F. , '' (1999 ) 29 Lett. Appl. Microbiol. : 130 -135 .

  • L.G.M. Gorris, K. Oosterhaven, K.J. Hartmans, J. De Witte , and E.J. Smid , Pests Dis. 3D-20 (1994) 307–312.

    Smid E.J. , '' (1994 ) 3D-20 Pests Dis. : 307 -312 .

  • M.V. Bh. Reddy, P. Angers, A. Gosselin , and J. Arul , Phytochemistry 47 (1998) 1515–1520.

    Arul J. , '' (1998 ) 47 Phytochemistry : 1515 -1520 .

  • C.C. Thanassoulopoulos and J. Laidou , Acta Hortic. 444 (1997) 757–764.

    Laidou J. , '' (1997 ) 444 Acta Hortic. : 757 -764 .

  • R.R. Goodall and A.A. Levi , Nature London 158 (1946) 675.

    Levi A.A. , '' (1946 ) 158 Nature London : 675 -.

  • R. Fischer and H. Lautner , Arch. Pharm., Weinheim 294 (1961) I.

    Lautner H. , '' (1961 ) 294 Arch. Pharm., Weinheim : I -.

  • L. Botz, S. Nagy , and B. Kocsis , in: Sz. Nyiredy (Ed.) Planar Chromatography, Springer, 2001, pp. 489–516.

  • B.M. Lund and G.D. Lyon , J. Chromatogr. 110 (1975) 193–196.

    Lyon G.D. , '' (1975 ) 110 J. Chromatogr. : 193 -196 .

  • A.J. Slusarenko, A. C. Longland , and I.M. Whitehead , Bot. Helv. 99 (1989) 203.

    Whitehead I.M. , '' (1989 ) 99 Bot. Helv. : 203 -.

  • European Pharmacopoeia, 4th edn, Druckerei C.H. Beck, Noördlingen, 2002, p. 183.

  • H. Wagner, S. Bladt , and E.M. Zgainski , Drogenanalyse, Dünnschichtschromatographische Analyse von Arzneidrogen, Springer, Berlin, 1983, p. 40.

    Zgainski E.M. , '', in Drogenanalyse, Dünnschichtschromatographische Analyse von Arzneidrogen , (1983 ) -.
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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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