The chromatographic separation of fenbufen, ibuprofen, ketoprofen, diclofenac sodium, mefenamic acid, and tiaprofenic acid has been investigated. Normal-phase chromatography on silica gel by the ascending and horizontal techniques, and reversed-phase chromatography on octadecyl-bonded silica gel (RP-18) in horizontal chambers, were performed with suitable mobile phases. The substances were identified by UV illumination at
= 254 nm and by use of dyeing reagents. Reversed phase chromatography with phosphate buffer, pH 5.73–10% CTMA-Br in methanol, 3.5 + 6.5 (
), as mobile phase enabled better separation of the six drugs than normal-phase mode. A simple videodensitometric TLC method on silica gel RP-18 was developed and validated for quantitative determination of fenbufen in tablets. The limits of detection and quantification were determined by videodensitometry at
= 254 nm. A calibration plot was constructed in the range 2.0–12.0 μg/5-μL spot and was linear with a good correlation coefficient (0.9926).
for quantitation of fenbufen were from 2.44 to 3.10%. The method was applied satisfactorily to pharmaceutical preparations.
, Introduction to Medicinal Chemistry, Wiley-VCH, New York, 1997.
Gringauz A. , '', in Introduction to Medicinal Chemistry, (1997) -.
Gringauz A. Introduction to Medicinal Chemistry1997)| false
T. Saito, S. Takeichi, Y. Nakajima, N. Yukawa
, J. Anal. Toxicol.
Osawa M. , '' (1997) 21J. Anal. Toxicol.: 584-586.
Osawa M. J. Anal. Toxicol.199721584586)| false
G. Thuku Thiongo
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Thuku Thiongo G. , '' (1999) 13Biomed. Chromatogr.: 276-278.
Thuku Thiongo G. Biomed. Chromatogr.199913276278)| false
, Acta Chromatogr.