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  • 1 Hungarian Academy of Sciences Plant Protection Institute Budapest, Herman O. Str. 15 H-1022 Budapest Hungary
  • 2 L. Eötvös University Department of Chemical Technology and Environmental Chemistry P.O.B. 32 H-1538 Budapest Hungary
  • 3 Ministry of Agriculture Research Institute for Viticulture and Enology, Experimental Wine Cellar Maláta u. 4 H-1105 Budapest Hungary
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New results with BioArena as a complex separation and detection (evaluation) system support earlier observations that formaldehyde (HCHO) and its reaction products play a special and crucial role in the effects of antibiotic in general. It has been established that antibiotic-like compounds (e.g. trans -resveratrol, Cu(II) ions) have a duplicate inhibiting effect on pathogen cells as a result of the action of HCHO. HCHO as a key molecule of the formaldehydome participates in series of interactions which can be screened by means of different spectroscopic and chromatographic techniques; accumulation of HCHO and its reaction products in TLC spots is limited, however, so indirect detection is advantageous. In BioArena the planar stationary phase bed after TLC/HPTLC and, mainly, OPLC separation can be used for manifestation of deprivation of HCHO from antibiotic spots, for observation of the duplicate effect of substances with a direct effect, and for demonstration of cell proliferation promotion and retardation.

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