Plant extracts from
Mill. contain tropane alkaloids which are pharmacologically active, and compounds used in medicine are isolated industrially from the plant. Control of the composition and content of these alkaloids is, therefore, an important task. Thin-layer chromatography is an effective method for analysis of these compounds, because they are difficult to detection with UV light. In the work discussed in this paper separation of closely related alkaloids was achieved by two-dimensional TLC in optimized NP and RP systems by use of multiphase plates. The work enabled identification of tropane alkaloids present in plant extracts. Normal-phase separation on silica layers was optimized for quantitative analysis of the alkaloids scopolamine and tropine and the sum of atropine and homatropine.
, Acta Polonia Pharm.
Grynkiewicz G. , '' (2001) 58Acta Polonia Pharm.: 481-492.
Grynkiewicz G. Acta Polonia Pharm.200158481492)| false
, J. Chromatogr. A.
Dräger B. , '' (2002) 978J. Chromatogr. A.: 1-35.
Dräger B. J. Chromatogr. A.2002978135)| false
O.L. Tombesi, B.E. Maldoni, E.R. Bartolome, H.M. Haurie
, J. Planar Chromatogr.