Preparative separation and isolation of coumarins from petroleum ether and methanol extracts of the fruits and roots of
L. Koch ex DC (
) have been achieved by column chromatography and normal- and reversed-phase TLC with gradient elution. Isolated coumarins were identified by comparison with standards in analytical TLC and in RP HPLC with gradient elution, by melting point (m.p.) measurement, by UV spectroscopy, by MS, and by
C NMR spectroscopy. Esculin, umbeliferone, bergapten, xanthotoxin, isoimperatorin, imperatorin, psoralen, and
-kellactone were isolated for the first time from
L. Koch ex DC. Two angular-type pyranocoumarins isolated from the roots and fruits of
L. Koch ex DC. were identified as pteryxin and epoxypteryxin by MS and
R.D.H. Murray, J. Mendez
. The Natural Coumarins: Occurrence, Chemistry and Biochemistry, Wiley, Chichester, 1982.
Brown S.A. , '', in The Natural Coumarins: Occurrence, Chemistry and Biochemistry, (1982) -.
Brown S.A. The Natural Coumarins: Occurrence, Chemistry and Biochemistry1982)| false
, Appl. Biol. Commun.
Gawron A. , '' (1991) 1/2Appl. Biol. Commun.: 85-90.
Gawron A. Appl. Biol. Commun.19911/28590)| false
, Illustrierte Flora von Mitteleuropa Band V Teil 2. Berlin, Hamburg, 1975.
, Herba Pol.
,3–4 (1984) 159–163.
Cisowski W. , '' (1984) 30Herba Pol.: 159-163.
Cisowski W. Herba Pol.198430159163)| false
J. Zielińska-Jenczylik, A. Sypula
, Arch. Immunol. Ther. Exp. Warsaw
(5) (1984) 577–582.