JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 18: Issue 104
Quantification of eugenol in Cinnamomum tamala nees and eberm. Leaf powder by high-performance thin-layer chromatography
Authors:
Vidya Dighe TDM Laboratory Plot No. 194, Scheme No. 6, Road No. 15 Sion (E), Koliwada, Mumbai-22 India

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Atish Gursale TDM Laboratory Plot No. 194, Scheme No. 6, Road No. 15 Sion (E), Koliwada, Mumbai-22 India

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Ramesh Sane TDM Laboratory Plot No. 194, Scheme No. 6, Road No. 15 Sion (E), Koliwada, Mumbai-22 India

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Sasikumar Menon TDM Laboratory Plot No. 194, Scheme No. 6, Road No. 15 Sion (E), Koliwada, Mumbai-22 India

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Shvetali Raje TDM Laboratory Plot No. 194, Scheme No. 6, Road No. 15 Sion (E), Koliwada, Mumbai-22 India

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Pages:
305–307
Online Publication Date:
17 Mar 2007
Publication Date:
01 Aug 2005
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.18.2005.4.10
Keywords:
Eugenol; Cinnamomum tamala Nees and Eberm; HPTLC; Quantitation
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Eugenol is used as a flavor in the food industry, has a variety of biological activity, and can serve as a biomarker. Because eugenol is present in the leaves of Cinnamomum tamala Nees and Eberm., which are used as a spice, a sensitive and reliable quantitative high-performance thin-layer chromatographic method has been established for quantification of the compound in the leaves of the plant. A methanol extract of the powder of dried leaves of Cinnamomum tamala Nees and Eberm. was applied to silica gel 60 F 254 TLC plates and these were developed with toluene-ethyl acetate-formic acid, 90 + 10 + 01 ( v/v ), as mobile phase. Detection and quantitation was performed by densitometry at λ = 280 nm. The accuracy of the method was checked by conducting recovery studies for two different levels of eugenol; the average recovery was found to be 98.39%. The average eugenol content, as estimated by use of the proposed method, was 5.405 mg g −1 . The HPTLC method proposed for the quantitative monitoring of eugenol in Cinnamomum tamala leaf powder is rapid, simple, and precise.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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