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  • 1 University of Applied Sciences Offenburg Badstrasse 24 77652 Offenburg Germany
  • | 2 University of Freiburg Institute of Legal Medicine University Hospital Freiburg Albertstrasse 9 79104 Freiburg Germany
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Diode-array planar chromatography is a versatile tool for identification of pharmaceutical substances In this paper thirty-three compounds with benzodiazepine properties were investigated and the separating conditions for silica gel HPTLC plates and three mobile phases were optimized. Diode-array HPTLC makes it possible to identify all the compounds with high certainty down to a level of 20 ng. An algorithm for spectral recognition which is combined with RF values from the three separation steps into one fit factor is presented. This set of data is unique for each of the compounds investigated and enables unequivocal identification. The method is rapid, inexpensive, and sensitive down to a level of 20 ng mL −1 .

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