Fluoroquinolones are a relatively new group of synthetic antibiotics (chemotherapeutics) widely used both in human and animal treatment. Because veterinary enrofloxacin and its main metabolite ciprofloxacin, a widely used human antibiotic, can be present as residues in milk, there is a need for methods for their separation and determination. Matrix solid-phase dispersion (MSPD) on a siliceous sorbent was used for isolation and concentration of enrofloxacin and ciprofloxacin residues from cows’ milk. This preseparation method was combined with high-performance liquid chromatography and with thin-layer chromatography-direct bioautography. Mean recoveries were calculated for different levels of the antibiotics in milk. The results revealed that thin-layer chromatography-direct bioautography can be used for the screening of enrofloxacin and ciprofloxacin at the maximum residue level stipulated for milk by the European Union.
J.A. Hernández-Arteseros, J. Barbosa, R. Compaňó
, J. Chromatogr. A
Prat M.D. , '' (2002) 945J. Chromatogr. A: 1-24.
Prat M.D. J. Chromatogr. A2002945124)| false
K.L. Tyczkowska, R.D. Voyksner, L.A. Kevin
, J. Chromatogr. B