JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 19: Issue 108
Screening of enrofloxacin and ciprofloxacin residues in milk by HPLC and by TLC with direct bioautography
Author:
Irena Choma M. Curie-Skłodowska University Department of Chromatographic Methods M. Skłodowska Sq. 3 20-031 Lublin Poland

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Pages:
104–108
Online Publication Date:
11 Mar 2007
Publication Date:
01 Apr 2006
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.19.2006.2.3
Keywords:
Enrofloxacin; Ciprofloxacin; HPLC; TLC-direct bioautography; Matrix solid-phase dispersion
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Fluoroquinolones are a relatively new group of synthetic antibiotics (chemotherapeutics) widely used both in human and animal treatment. Because veterinary enrofloxacin and its main metabolite ciprofloxacin, a widely used human antibiotic, can be present as residues in milk, there is a need for methods for their separation and determination. Matrix solid-phase dispersion (MSPD) on a siliceous sorbent was used for isolation and concentration of enrofloxacin and ciprofloxacin residues from cows’ milk. This preseparation method was combined with high-performance liquid chromatography and with thin-layer chromatography-direct bioautography. Mean recoveries were calculated for different levels of the antibiotics in milk. The results revealed that thin-layer chromatography-direct bioautography can be used for the screening of enrofloxacin and ciprofloxacin at the maximum residue level stipulated for milk by the European Union.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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