JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 19: Issue 108
Use of DryLab for simulation of TLC separation and method transfer from TLC to HPLC
Authors: Tero Wennberg 1 , Irena Vovk 2 , Pia Vuorela 3 , Breda Simonovska 2 , and Heikki Vuorela 1
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  • 1 University of Helsinki Division of Pharmaceutical Biology, Faculty of Pharmacy P.O. Box 56 FIN-00014 Helsinki Finland
  • 2 National Institute of Chemistry Laboratory for Food Chemistry Hajdrihova 19 SI-1000 Ljubljana Slovenia
  • 3 Åbo Akademi University Department of Biochemistry and Pharmacy BioCity FIN-20520 Turku Finland
DOI:
https://doi.org/10.1556/jpc.19.2006.2.6
Page Count:
118–123
Publication Date:
01 Apr 2006
Online Publication Date:
11 Mar 2007
Article Category:
Research Article
Restricted access

The computer-assisted simulation program DryLab has been used to simulate TLC separations. The simulations were based on data from preliminary TLC separations. For DryLab data entry RF values from TLC were converted to retention times, the development distance on the plate was used as column length, and the plate thickness was used as the column diameter. To achieve reasonably accurate simulations it was found necessary to run three preliminary runs in which differences between organic modifier concentration in two adjacent runs was more than 5%. The possibility of predicting HPLC separation on the basis of TLC separations was also studied. It was found that the method can be transferred from TLC to HPLC and that DryLab can be used to predict HPLC separation on the basis of the information obtained from TLC experiments. To produce a reasonably accurate HPLC simulation on the basis of TLC data, however, a relatively large number of preliminary experiments is required.

  • F. Geiss , Fundamentals of Thin Layer Chromatography (Planar Chromatography), Hüthig, Heidelberg, 1987.

    Geiss F. , '', in Fundamentals of Thin Layer Chromatography (Planar Chromatography) , (1987 ) -.

  • S. Reuke and H.E. Hauck , Fresenius’ Z. Anal. Chem. 351 (1995) 739–744.

    Hauck H.E. , '' (1995 ) 351 Fresenius’ Z. Anal. Chem. : 739 -744.

  • J.K. Rózylo and M. Janicka , J. Planar Chromatogr. 4 (1991) 241–245.

    Janicka M. , '' (1991 ) 4 J. Planar Chromatogr. : 241 -245.

  • J.K. Rózylo and M. Janicka , J. Planar Chromatogr. 9 (1996) 418–424.

    Janicka M. , '' (1996 ) 9 J. Planar Chromatogr. : 418 -424.

  • P. Vuorela, E.-L. Rahko, R. Hiltunen , and H. Vuorela , J. Chromatogr. A 670 (1994) 191–198.

    Vuorela H. , '' (1994 ) 670 J. Chromatogr. A : 191 -198.

  • W. Golkiewicz , Chromatographia 14 (1981) 629–632.

    Golkiewicz W. , '' (1981 ) 14 Chromatographia : 629 -632.

  • G.C. Kiss, E. Forgács, T. Csetháti , and J.A. Vizcaino , J. Chromatogr. A 896 (2000) 61–68.

    Vizcaino J.A. , '' (2000 ) 896 J. Chromatogr. A : 61 -68.

  • H.J. Issaq, B. Shaikh, N.J. Pontzer , and E.W. Barr , J. Liq. Chromatogr. 1 (2) (1978) 133–149.

    Barr E.W. , '' (1978 ) 1 J. Liq. Chromatogr. : 133 -149.

  • W. Jost, H.E. Hauck , and F. Eisenbeib , Fresenius’ Z Anal. Chem. 318 (1984) 300–301.

    Eisenbeib F. , '' (1984 ) 318 Fresenius’ Z Anal. Chem. : 300 -301.

  • H. Jork and B. Roth , J. Chromatogr. 144 (1977) 39–56.

    Roth B. , '' (1977 ) 144 J. Chromatogr. : 39 -56.

  • W. Golkiewicz , Chromatographia 14 (1981) 411–414.

    Golkiewicz W. , '' (1981 ) 14 Chromatographia : 411 -414.

  • E. Von Arx and M. Faupel , J. Chromatogr. 154 (1978) 68–72.

    Faupel M. , '' (1978 ) 154 J. Chromatogr. : 68 -72.

  • I. Molnar , J. Chromatogr. A 965 (2002) 175–194.

    Molnar I. , '' (2002 ) 965 J. Chromatogr. A : 175 -194.

  • J.K. Rózylo, M. Janicka , and R. Siembida , J. Liq. Chromatogr. 17 (17) (1994) 3641–3653.

    Siembida R. , '' (1994 ) 17 J. Liq. Chromatogr. : 3641 -3653.

  • M. Janicka and J.K. Rózylo , J. Planar Chromatogr. 6 (1993) 362–367.

    Rózylo J.K. , '' (1993 ) 6 J. Planar Chromatogr. : 362 -367.

  • T. Tuzimski and E. Soczewinski , Chromatogr. 56 (2002) 225–227.

    Soczewinski E. , '' (2002 ) 56 Chromatogr. : 225 -227.

  • J.K. Rózylo and M. Janicka , J. Liq. Chromatogr. 14 (16/17) (1991) 3197–3212.

    Janicka M. , '' (1991 ) 14 J. Liq. Chromatogr. : 3197 -3212.

  • M. Janicka and J.K. Rózylo , J. Planar Chromatogr. 3 (1990) 413–416.

    Rózylo J.K. , '' (1990 ) 3 J. Planar Chromatogr. : 413 -416.
Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993
Keywords:
TLC; HPLC; DryLab; Optimization; Method transfer

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