The dried fruits of Piper longum are extensively used in Ayurvedic medicinal preparations. A simple and convenient HPTLC method has been developed for standardization of the plant material using the two major constituents, pellitorine and dihydropiperlonguminine, as markers. The stationary phase was silica gel 60 F 254 , hexane-ethyl acetate, 75 + 25 ( v/v ), was used as mobile phase, and detection was at λ = 260 nm. The method is characterized by high sensitivity and linearity over a wide range of concentrations. The results obtained were evaluated statistically.
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