View More View Less
  • 1 Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247 667 India
Restricted access

A simple and sensitive method for separation and quantitative determination of antidiabetic drugs in pharmaceutical preparations has been established and validated. Commercial formulations of five antidiabetic drugs (metformin, pioglitazone, rosiglitazone, glibenclamide, and gliclazide) were chosen for the studies. The compounds were extracted, isolated, purified, recrystallized, and characterized by measurement of melting point, λmax , and IR. Quantitative determination was performed by HPLC, TLC, and column chromatography supplemented with UV spectrophotometry. Two of the combinations, metformin + pioglitazone and metformin + gliclazide, were separated by open-column chromatography. Detection was by UV spectrophotometry in HPLC and by use of iodine vapor in TLC.

  • S. AbuRuz, J. Millership , and J. McElnay , J. Chromatogr. B 817 (2005) 277–286.

    McElnay J. , '' (2005 ) 817 J. Chromatogr. B : 277 -286.

  • S. AbuRuz, J. Millership , and J. McElnay , J. Chromatogr. B 798 (2003) 203–209.

    McElnay J. , '' (2003 ) 798 J. Chromatogr. B : 203 -209.

  • H.Y. Kah and K.P. Kok , J. Chromatogr. B 710 (1998) 243–246.

    Kok K.P. , '' (1998 ) 710 J. Chromatogr. B : 243 -246.

  • A. Zarghi, S.M. Foroutan, A. Shafaati , and A. Khoddam , J. Pharm. Biomed. Anal. 31 (2003) 197–200.

    Khoddam A. , '' (2003 ) 31 J. Pharm. Biomed. Anal. : 197 -200.

  • C.-L. Cheng and C.-H. Chou , J. Chromatogr. B 762 (2001) 51–58.

    Chou C.-H. , '' (2001 ) 762 J. Chromatogr. B : 51 -58.

  • R. Huupponen, P. Ojala-Karlsson, J. Rouru , and M. Koulu , J. Chromatogr. 583 (1992) 270–273.

    Koulu M. , '' (1992 ) 583 J. Chromatogr. : 270 -273.

  • J. Keal and A. Somogyi , J. Chromatogr. 378 (1986) 503–508.

    Somogyi A. , '' (1986 ) 378 J. Chromatogr. : 503 -508.

  • M. Ohta, M. Iwasaki, M. Kai , and Y. Ohkura , Anal. Sci. 9 (1993) 217–230.

    Ohkura Y. , '' (1993 ) 9 Anal. Sci. : 217 -230.

  • J.-Z. Song, H.-F. Chen, S.-J. Tian , and Z.-P. Sun , J. Chromatogr. B 708 (1998) 277–283.

    Sun Z.-P. , '' (1998 ) 708 J. Chromatogr. B : 277 -283.

  • Y. Wang, Y. Tang, J. Gu, J.P. Fawcett , and X. Bai , J. Chromatogr. B 808 (2004) 215–219.

    Bai X. , '' (2004 ) 808 J. Chromatogr. B : 215 -219.

  • S. Ashour and R. Kabbani , Anal. Lett. 36 (2003), 361–370.

    Kabbani R. , '' (2003 ) 36 Anal. Lett. : 361 -370.

  • S.S.M. Hassan, W.H. Mahmoud, M.A.F. Elmosallamy , and A.H.M. Othman , Anal. Chim. Acta 378 (1999) 299–311.

    Othman A.H.M. , '' (1999 ) 378 Anal. Chim. Acta : 299 -311.

  • T. Radhakrishna, R.D. Sreenivas , and G.R. Om , J. Pharm. Biomed. Anal. 29 (2002) 593–607.

    Om G.R. , '' (2002 ) 29 J. Pharm. Biomed. Anal. : 593 -607.

  • K. Yamashita, H. Murakami, T. Okuda , and M. Motohashi , J. Chromatogr. B 671 (1996) 141–146.

    Motohashi M. , '' (1996 ) 671 J. Chromatogr. B : 141 -146.

  • W.Z. Zhong and D.B. Lakings , J. Chromatogr. 490 (1989) 377–381.

    Lakings D.B. , '' (1989 ) 490 J. Chromatogr. : 377 -381.

  • A. Gumieniczek, H. Hopkala , and A. Berecka , J. Liq. Chromatogr. Related Technol. 27 (2004) 2057–2070.

    Berecka A. , '' (2004 ) 27 J. Liq. Chromatogr. Related Technol. : 2057 -2070.

  • M.R.T. Sane, S. Inamdar, M. Mandar , and A. Menezes , J. Planar Chromatogr. 17 (2004) 154–157.

    Menezes A. , '' (2004 ) 17 J. Planar Chromatogr. : 154 -157.

  • Z.J. Lin, W. Ji, D. Desai-Krieger , and L. Shum , J. Pharm. Biomed. Anal. 33 (2003) 101–108.

    Shum L. , '' (2003 ) 33 J. Pharm. Biomed. Anal. : 101 -108.

  • D.G Sankar, J.M.R. Kumar , and M.V.V.N. Reddy , Asian J. Chem. 16 (2004) 251–255.

    Reddy M.V.V.N. , '' (2004 ) 16 Asian J. Chem. : 251 -255.

  • R.N.V.S Mamidi, B. Benjamin, M. Ramesh , and N.R. Srinivas , Biomed. Chromatogr. 17 (2003) 417–420.

    Srinivas N.R. , '' (2003 ) 17 Biomed. Chromatogr. : 417 -420.

  • T. Radhakrishna, J. Satyanarayana , and A. Satayanarayana , J. Pharm. Biomed. Anal. 29 (2002) 873–880.

    Satayanarayana A. , '' (2002 ) 29 J. Pharm. Biomed. Anal. : 873 -880.

  • P. Gomes, J. Sippel, A. Jablonski , and M. Steppe , J. Pharm. Biomed. Anal. 36 (2004) 909–913.

    Steppe M. , '' (2004 ) 36 J. Pharm. Biomed. Anal. : 909 -913.

  • K.-H. Kim and J. Park , Biomed. Chromatogr. 18 (2004) 613–615.

    Park J. , '' (2004 ) 18 Biomed. Chromatogr. : 613 -615.

  • R.T. Sane, M. Francis, A. Moghe, S. Khedkar , and A. Anerao , J. Planar Chromatogr. 15 (2002) 192–194.

    Anerao A. , '' (2002 ) 15 J. Planar Chromatogr. : 192 -194.

  • A. Gumieniczek, A. Berecka, H. Hopkala , and T. Mroczek , J. Liq. Chromatogr. Related Technol. 26 (2003) 3307–3311.

    Mroczek T. , '' (2003 ) 26 J. Liq. Chromatogr. Related Technol. : 3307 -3311.

  • E.N.M. Ho, K.C.H. Yiu, T.S.M. Wan, B.D. Stewart , and K.L. Watkins , J. Chromatogr. B 811 (2004) 65–73.

    Watkins K.L. , '' (2004 ) 811 J. Chromatogr. B : 65 -73.

  • M.R. Rouini, A. Mohajer , and M.H. Tahami , J. Chromatogr. B 785 (2003) 383–386.

    Tahami M.H. , '' (2003 ) 785 J. Chromatogr. B : 383 -386.

  • F.K. Glowka and T.W. Hermann , Chem. Anal. 42 (1997) 215–218. AN:255284 (1997).

    Hermann T.W. , '' (1997 ) 42 Chem. Anal. : 215 -218.

  • H. Noguchi, N. Tomita, S. Natuto , and S. Nakano , J. Chromatogr. B 583 (1992) 266–267.

    Nakano S. , '' (1992 ) 583 J. Chromatogr. B : 266 -267.

  • A. Igaki, K. Kobayashi, M. Kimura, T. Sakoguchi , and A. Matsuoka , J. Chromatogr. 493 (1989) 222–229.

    Matsuoka A. , '' (1989 ) 493 J. Chromatogr. : 222 -229.

  • J. Krzek, M. Dabrowska , and U. Hubicka , J. Planar Chromatogr. 14 (2001) 183–187.

    Hubicka U. , '' (2001 ) 14 J. Planar Chromatogr. : 183 -187.

  • N. El-Enany , Farmaco 59 (2004) 63–69.

    El-Enany N. , '' (2004 ) 59 Farmaco : 63 -69.

  • B.L. Kolte, B.B. Raut, A.A. Deo, M.A. Bagool , and D.B. Shinde , J. Liq. Chromatogr. Related Technol. 26 (2003) 1117–1133.

    Shinde D.B. , '' (2003 ) 26 J. Liq. Chromatogr. Related Technol. : 1117 -1133.

  • J. Krzek, J. Czekaj, M. Momiczewska , and W. Rzeszutko , J. AOAC Int. 84 (2001) 1695–1702.

    Rzeszutko W. , '' (2001 ) 84 J. AOAC Int. : 1695 -1702.

  • T. Maeda, T. Yamaguchi , and M. Hashimoto , J. Chromatogr. 223 (1981) 357–363.

    Hashimoto M. , '' (1981 ) 223 J. Chromatogr. : 357 -363.

  • G. Linder, L. Herbertz , and H. Reinauer , Laboratoriumsmsmedizin 4 (1980) 34–37; AN: 542558 (1980).

    Reinauer H. , '' (1980 ) 4 Laboratoriumsmsmedizin : 34 -37.

  • J.V. Santurio and E.G. Porto , J. Chromatogr. B 682 (1996) 364–370.

    Porto E.G. , '' (1996 ) 682 J. Chromatogr. B : 364 -370.

  • I. Niopas and A.C. Daftsios , J. Pharm. Biomed. Anal. 28 (2002) 653–357.

    Daftsios A.C. , '' (2002 ) 28 J. Pharm. Biomed. Anal. : 653 -357.

  • A. Khamis, I. Khalil, E. Sayed, M. Yousry, A. Khalid , and A.M. Yamani , Anal. Lett. 27 (1994) 1277–1279. AN: 449518 (1994).

    Yamani A.M. , '' (1994 ) 27 Anal. Lett. : 1277 -1279.

  • M.A.M. Shehata, M.Y.Y. Mohamed , and B.A. Abdel , Bull. Fac. Pharm. 38 (2000) 7–11. AN: 14567 (2001).

    Abdel B.A. , '' (2000 ) 38 Bull. Fac. Pharm. : 7 -11.

  • A. Jonkowski, A. Marzec, A. Shoreka , and H. Lamparczyk , Acta Chromatogr. 2 (1993) 33–39; AN:620913 (1994).

    Lamparczyk H. , '' (1993 ) 2 Acta Chromatogr. : 33 -39.

  • P.G. Takla , and S.R. Joshi , J. Pharm. Biomed. Anal. 1 (1983) 189–192.

    Joshi S.R. , '' (1983 ) 1 J. Pharm. Biomed. Anal. : 189 -192.

  • C.L. Andrews, C.-P. Yu, E. Yang , and P. Vouros , J Chromatogr A 1053 (2004) 151–159.

    Vouros P. , '' (2004 ) 1053 J Chromatogr A : 151 -159.

  • B.-M. Chen, Y.-Z. Liang, F.-Q. Guo, L.-F. Huang, F.-L. Deng, X. Chen , and Y.-L Wang , Anal. Chim. Acta 514 (2004) 185–191.

    Wang Y.-L. , '' (2004 ) 514 Anal. Chim. Acta : 185 -191.

  • ICH Harmonised Tripartite Guideline Q2B, Validation of Analytical Procedures: Methodology, 1996.

  • M. Thompson, L. R. Stephen , and R. Wood , Pure Appl. Chem. 74 (2002) 835 (IUPAC technical report).

    Wood R. , '' (2002 ) 74 Pure Appl. Chem. : 835 -.

All Time Past Year Past 30 Days
Abstract Views 130 130 9
Full Text Views 23 4 0
PDF Downloads 19 1 0