Authors:
Nazli Nader Shaheed Beheshti University of Medical Sciences Traditional Medicine and Materia Medica Research Center P.O. Box 14155-6354 Tehran Iran

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Somayeh Esmaeili Shaheed Beheshti University of Medical Sciences Traditional Medicine and Materia Medica Research Center P.O. Box 14155-6354 Tehran Iran

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Farzaneh Naghibi Shaheed Beheshti University of Medical Sciences Traditional Medicine and Materia Medica Research Center P.O. Box 14155-6354 Tehran Iran

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Mahmoud Mosaddegh Shaheed Beheshti University of Medical Sciences Traditional Medicine and Materia Medica Research Center P.O. Box 14155-6354 Tehran Iran

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A simple, rapid, and sensitive high-performance thin-layer chromatographic method has been developed for determination of apigenin in liquid products of Matricaria chamomilla L. (syn. Chamomilla recutita L.). Samples were applied directly to silica gel 60F 254 HPTLC plates as stationary phase. Plates were developed to a distance of 90 mm in a glass twin-trough chamber with toluene-methanol, 10 + 2 ( v/v ), as mobile phase. The plates were scanned densitometrically at λ = 343 nm. The method was validated for selectivity, repeatability, and accuracy. The repeatability of the method was excellent with a mean RSD of 2.2%. The accuracy was checked by studying recovery at two levels and average recovery was 101.4%. The method was confirmed to be suitable for determination of apigenin in Matricaria chamomilla liquid products.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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