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  • 1 Medical University Department of Pharmaceutical Botany 1 Chodżki Street 20-093 Lublin Poland
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In the 5th European Pharmacopoeia a thin-layer chromatographic (TLC) technique based on analysis of ascorbic acid only is stipulated for qualitative analysis of Rosae pseudofructus, a drug from the dog rose. In this paper a new, simple method is proposed in which the TLC pattern of the flavonoid content is used for effective and reliable quality control of the drug. The method can also be used for identification of different Rosa L. species. The proposed assay uses ultrasound-assisted extraction then clean-up by solid-phase extraction before chromatographic analysis. Quercetin 3-rhamnoside, quercetin 3-glucoside, quercetin 3-rutinoside, catechin, and gallic acid are used as markers. The results reveal that the polyphenol composition of rose pseudo-fruits furnishes a specific TLC chromatogram fingerprint which might be helpful for quality assurance and detection of adulteration of crude extracts.

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