JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 20: Issue 1
HPTLC determination of ursolic acid in Alstonia scholaris R. Br.
Authors:
Prabha Shetty S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

Search for other papers by Prabha Shetty in
Current site
Google Scholar
PubMed Close
,
Kiran Mangaonkar Shri Vile Parle Kelvani Mandal’s Mithibai College Mumbai 400 056 India

Search for other papers by Kiran Mangaonkar in
Current site
Google Scholar
PubMed Close
, and
Ramesh Sane Guru Nanak Institute for Research and Development Mumbai 400 019 India

Search for other papers by Ramesh Sane in
Current site
Google Scholar
PubMed Close
Pages:
65–68
Online Publication Date:
07 Mar 2007
Publication Date:
01 Feb 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.20.2007.1.11
Keywords:
HPTLC; Standardization; Alstonia scholaris R. Br; Ursolic acid
Restricted access

Among the complex mixture of biologically active compounds in the leaves of Alstonia scholaris , a plant used in traditional medicine, ursolic acid, a constituent of the leaves, has been used as an analytical marker indicative of the quality of the plant. A sensitive thin-layer chromatographic method has been developed for determination of ursolic acid in the leaves of Alstonia scholaris . Chromatographic separation was performed on silica gel HPTLC plates with toluene-ethyl acetate-triethylamine-methanol, 7 + 2 + 1 + 1 ( v/v ), as mobile phase. The plates were scanned densitometrically at λ = 366 nm. The method was validated for precision, repeatability, and accuracy. Intra-day and inter-day RSD were 0.43% and 1.54%, respectively. Instrumental precision and repeatability of the method were found to be 0.54 and 1.20 (% CV), respectively. The accuracy was checked by studying recovery at three levels; average recovery was 100.26%. The method proposed for quantitative monitoring of ursolic acid in Alstonia scholaris is rapid, simple, and accurate and can be used for routine quality testing.

  • R.N. Chopra, S.L. Nayar , and I.C. Chopra , Glossary of Indian medicinal plants, CSIR, New Delhi, 1956.

    Chopra I.C. , '', in Glossary of Indian medicinal plants , (1956 ) -.

  • K.R. Kirtikar and B.D. Basu , Indian Medicinal plants. Vol. III, L.M. Basu Publications, Allahabad, 1935.

    Basu B.D. , '', in Indian Medicinal plants. Vol. III , (1935 ) -.

  • N. Valecha, C.U. Devi , and H. Joshi , Curr. Sci. 78 (2000) 1120.

    Joshi H. , '' (2000 ) 78 Curr. Sci. : 1120 -.

  • Medicinal Plants of India, I.C.M.R., New Delhi, 1975.

  • Avijit Banerji and Julie Banerji , Indian J. Chem. 15B (1977) 390–391.

    Banerji J. , '' (1977 ) 15B Indian J. Chem. : 390 -391 .

  • D.N. Dhar, S.C. Suri , and P. Dwiwedi , Planta Med. 31 (1977) 33–34.

    Dwiwedi P. , '' (1977 ) 31 Planta Med. : 33 -34 .

  • L Jie , J. Ethnopharmacol. 49 (1995) 57–68.

    Jie L. , '' (1995 ) 49 J. Ethnopharmacol. : 57 -68 .

  • V. Rao, V. Gopalkrishnan , and K. Rajandra Prasad , Indian Drugs 38 (2001) 216–224.

    Rajandra Prasad K. , '' (2001 ) 38 Indian Drugs : 216 -224 .

  • D. Baricevic, S. Sosa, D. Loggia, A. Tubaro, B. Simonovska, A. Krasna , and A. Zupancic , J. Ethnopharmacol. 75 (2001) 125–132.

    Zupancic A. , '' (2001 ) 75 J. Ethnopharmacol. : 125 -132 .

  • I.A. Murav’ev, V.V. Shatilo , and V.F. Semenchenko , Chem. Nat. Prod. 8 (1974) 720–721.

    Semenchenko V.F. , '' (1974 ) 8 Chem. Nat. Prod. : 720 -721 .

  • L. Lacikova, M.E. Abdel-Hamid, L. Novotny , et al. Chromatographia 63 (2006) 117–122.

    Novotny L. , '' (2006 ) 63 Chromatographia : 117 -122 .

  • L. Novotny, M.E. Abdel-Hamid, H. Hamza , et al. J. Pharm. Biomed. Anal. 31 (2003) 961–968.

    Hamza H. , '' (2003 ) 31 J. Pharm. Biomed. Anal. : 961 -968 .

  • L.H. Yang, L.H. Zhao , and D.N. Zhu , Zonh Guo Zong Yao Za Zhi 14 (1994) 30–32.

    Zhu D.N. , '' (1994 ) 14 Zonh Guo Zong Yao Za Zhi : 30 -32 .

  • S.M. Zhang, G. Hong , and J.M. Chen , Chin. J. Anal. Chem. 28 (2000) 129–131.

    Chen J.M. , '' (2000 ) 28 Chin. J. Anal. Chem. : 129 -131 .

  • X. T Yang, L.L Cheng , and K Mi , Shanghai Zhong Yi Yao Da Xue Xue Bao 15 (2001) 53–55.

    Mi K. , '' (2001 ) 15 Shanghai Zhong Yi Yao Da Xue Xue Bao : 53 -55 .

  • P.D. Sethi , High-Performance Thin-Layer Chromatographic Quantitative Analysis of Pharmaceutical Formulations, CBS, India, 1996.

    Sethi P.D. , '', in High-Performance Thin-Layer Chromatographic Quantitative Analysis of Pharmaceutical Formulations , (1996 ) -.

  • J.B. Harborne , Phytochem. Methods, Springer, Berlin, 2005.

    Harborne J.B. , '', in Phytochem , (2005 ) -.

  • W. Grimm , Drug Dev. Ind. Pharm. 24 (1998) 313–325.

    Grimm W. , '' (1998 ) 24 Drug Dev. Ind. Pharm. : 313 -325 .

  • S. Badami, M.K. Gupta, N. Mathew, S.N. Meyyanathan, B. Suresh , and D.J. Bendell , J. Planar Chromatogr. 15 (2002) 183–186.

    Bendell D.J. , '' (2002 ) 15 J. Planar Chromatogr. : 183 -186 .
  • Collapse
  • Expand
Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2024 34 0 0
Dec 2024 41 0 0
Jan 2025 17 0 0
Feb 2025 27 0 0
Mar 2025 47 0 0
Apr 2025 11 0 0
May 2025 0 0 0