Authors:
Krzysztof GrzywnowiczUniversity of Maria Curie-Skłodowska Dept. of Biochemistry Pl. M.C. Skłodowskiej 3 20-031 Lublin Poland

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Małgorzata NowickaUniversity of Maria Curie-Skłodowska Dept. of Biochemistry Pl. M.C. Skłodowskiej 3 20-031 Lublin Poland

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We have compared the profiles of toxigenic molds and their mycotoxins after collection of dust from work-place buildings by simple use of adhesive tape. After cultivation of the molds on a variety of agars, and selected molds in appropriate liquid media, mycelia, conidia, spores, and culture filtrates were collected, extracted with methanol, and the extracts were concentrated. The extracts were investigated by TLC on silica gel plates with chloroform-xylene-acetone, 6 + 3 + 1 ( v/v ), as mobile phase and, usually, UV detection. After theoretical simulation, and optimization of several systems, these conditions were selected because they furnished better separations and were highly reproducible, thus enabling comparison of results. The results obtained revealed it was possible to identify differences among species of toxigenic molds and most of the mycotoxins present in work places by use of TLC. Our results were indicative of differences among offices, student laboratories, instrumental laboratories, sterile or partly sterile rooms, and lecture rooms. After standardization, methods similar to this can be offered as kits for detection of mycotoxins in work places.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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