Plants of the
genus are perennial rhizomatus plants belonging to the Zingiberaceae family. Extracts of Zingiberacaea have long been used in traditional medicine. A simple, precise, and convenient HPTLC method has been established for analysis of hedychenone, the major marker compound extracted from the rhizomes of
(Buch-Hem). Chromatography was performed on silica gel 60F
plates with ethyl acetate-hexane, 20 + 80 (
), as mobile phase. Detection and quantification were performed densitometrically at
= 254 nm with hedychenone as external standard. The method is characterized by high sensitivity and linearity over a wide range of concentrations.
S.C. Sharma, J.S. Tandon, H. Uprety, Y.N. Shukla