View More View Less
  • 1 Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247 667 India
  • | 2 Justus-Liebig-University of Giessen Interdisciplinary Research Center (IFZ) Heinrich-Buff-Ring 26 35392 Giessen Germany
Restricted access

A simple and rapid method has been established for indirect separation of the optical isomers of seventeen DL amino acids by reversed-phase and normal-phase TLC. Amino acids derivatized with 1-fluoro-2,4-dinitrophenyl-5-L -alaninamide (FDNP-L -Ala-NH 2 ), 1-fluoro-2,4-dinitrophenyl-5-L -phenylalaninamide (FDNP-L -Phe-NH 2 ), or 1-fluoro-2,4-dinitrophenyl-5-L -valinamide (FDNP-L -Val-NH 2 ) were spotted on precoated plates. Diastereomers of all the DL amino acids were separated most effectively by normal-phase TLC with phenol-water, 3:1 ( v / v ), as mobile phase. In reversed-phase TLC the diastereomers were separated most effectively by use of mobile phases containing acetonitrile and triethylamine-phosphate buffer (50 mM, pH 5.5). The results obtained by use of the classical Marfey’s reagent (FDNP-L -Ala-NH 2 ) were compared with those obtained by use of FDNP-L -Phe-NH 2 and FDNP-L -Val-NH 2 . The effects of buffer concentration, pH, and concentration of organic modifier were studied. This indirect method enabled resolution of DL amino acids at nanomolar concentrations.

  • R. Bhushan and J. Martens In: J. Sherma and B. Fried (Eds) Handbook of Thin-Layer Chromatography, 3rd edn, Marcel Dekker, New York, 2003.

    Martens J. , '', in Handbook of Thin-Layer Chromatography , (2003 ) -.

  • R. Bhushan and J. Martens , J. Biomed. Chromatogr. 11 (5) (1997) 280–285.

    Martens J. , '' (1997 ) 11 J. Biomed. Chromatogr. : 280 -285.

  • J. Martens and R. Bhushan , J. Pharm. Biomed. Anal. 8 (3) (1990) 259–269.

    Bhushan R. , '' (1990 ) 8 J. Pharm. Biomed. Anal. : 259 -269.

  • K. Günther and K. Möller In: Handbook of Thin Layer Chromatography, 3rd edn, Marcel Dekker, New York, 1996.

    Möller K. , '', in Handbook of Thin Layer Chromatography , (1996 ) -.

  • L. Lepri, V. Coas, P.G. Desideri , and L. Pettini , J. Planar Chromatogr. 5 (1992) 364–367.

    Pettini L. , '' (1992 ) 5 J. Planar Chromatogr. : 364 -367.

  • L. Lepri, V. Coas, P.G. Desideri , and D. Santiani , Chromatographia 36 (1993) 297–301.

    Santiani D. , '' (1993 ) 36 Chromatographia : 297 -301.

  • D.W. Armstrong and Y. Zhou , J. Liq. Chromatogr. 17 (1994) 1695–1707.

    Zhou Y. , '' (1994 ) 17 J. Liq. Chromatogr. : 1695 -1707.

  • I. Malinowska and J.K. Rozylo , Biomed. Chromatogr. 11 (1997) 272–275.

    Rozylo J.K. , '' (1997 ) 11 Biomed. Chromatogr. : 272 -275.

  • R. Bhushan, G.P. Reddy , and S. Joshi , J. Planar Chromatogr. 7 (1994) 126–128.

    Joshi S. , '' (1994 ) 7 J. Planar Chromatogr. : 126 -128.

  • Q.Y. Deng, Z. Zhang, Y.F. Zhu , and J.Y. Su. Fenxi Huaxue 25 (1997) 197–200.

    Su J.Y. , '' (1997 ) 25 Fenxi Huaxue : 197 -200.

  • R. Bhushan, J. Martens, S. Wallbaum, S. Joshi , and V. Parshad , Biomed. Chromatogr. 11 (1997) 286–288.

    Parshad V. , '' (1997 ) 11 Biomed. Chromatogr. : 286 -288.

  • R. Bhushan, J. Martens , and G.T. Thiong’o , J. Pharm. Biomed. Anal. 21 (2000) 1143–1147.

    Thiong’o G.T. , '' (2000 ) 21 J. Pharm. Biomed. Anal. : 1143 -1147.

  • R. Bhushan and M. Arora , Biomed. Chromatogr. 15 (2001) 433–436.

    Arora M. , '' (2001 ) 15 Biomed. Chromatogr. : 433 -436.

  • R. Bhushan and S. Joshi , Biomed. Chromatogr. 7 (1993) 235–250.

    Joshi S. , '' (1993 ) 7 Biomed. Chromatogr. : 235 -250.

  • K.J. Ruterbories and D. Nurok , Anal. Chem. 59 (1987) 2735–2736.

    Nurok D. , '' (1987 ) 59 Anal. Chem. : 2735 -2736.

  • Y. Nagata, T. Iida , and M. Sakai , J. Mol. Catal. B 12 (2001) 105–108.

    Sakai M. , '' (2001 ) 12 J. Mol. Catal. B : 105 -108.

  • H. Brückner and C. Keller-Hoehl , Chromatographia 30 (1990) 621–629.

    Keller-Hoehl C. , '' (1990 ) 30 Chromatographia : 621 -629.

  • C. B’Hymer, M. Montes-Bayon , and J.A. Caruso , J. Sep. Sci. 26 (2003) 7–19.

    Caruso J.A. , '' (2003 ) 26 J. Sep. Sci. : 7 -19.

  • R. Bhushan and H. Brückner , Amino Acids 27 (2004) 231–247.

    Brückner H. , '' (2004 ) 27 Amino Acids : 231 -247.

  • H. Brückner and C.J. Gah , J. Chromatogr. 555 (1991) 81–95.

    Gah C.J. , '' (1991 ) 555 J. Chromatogr. : 81 -95.

  • P. Marfey , Carlsberg Res. Commun. 243 (1984) 591–596.

    Marfey P. , '' (1984 ) 243 Carlsberg Res. Commun. : 591 -596.

  • Aldrich Handbook of Fine Chemicals, 2003–2004.

  • T. Kowalska In: J. Sherma and B. Fried (Eds) Handbook of Thin-Layer Chromatography, 3rd edn, Marcel Dekker, New York, 2003.

    Kowalska T. , '', in Handbook of Thin-Layer Chromatography , (2003 ) -.

For Submission Guideline click HERE

Manuscript Submission: JPC Manuscript Submission

Senior editors

Editor(s)-in-Chief: B. Spangenberg

Managing Editor: Kakuk Ágnes

Editorial Board

  • T.H. Dzido (Lublin, Poland)
  • Á. M. Móricz (Budapest, Hungary)
  • C.F. Poole (Detroit, MI, USA)
  • E. Reich (Muttenz, Switzerland)
  • B. Renger (Radolfzell, Germany)
  • I. Vovk (Ljubljana, Slovenia)

 

Advisory Board

  • E. BODOKI (Cluj-Napoca, Romania)
  • U. A. TH. BRINKMAN (Amsterdam, The Netherlands)
  • I. CHOMA (Lublin, Poland)
  • V. COMAN (Cluj-Napoca, Romania)
  • W. DAMMERTZ (Kressbronn, Germany)
  • K. FERENCZI-FODOR (Budapest, Hungary)
  • G. INDRAYANTO (Surabaya, Indonesia)
  • R. E. KAISER (Bad Dürkheim, Germany)
  • I. KLEBOVICH (Budapest, Hungary)
  • T. KOWALSKA (Katowice, Poland)
  • L. LEPRI (Florence, Italy)
  • E. MINCSOVICS (Budapest, Hungary)
  • D. NUROK (Indianapolis, IN, USA)
  • B. RENGER (Radolfzell, Germany)
  • J. K. RÓŻYŁO (Lublin, Poland)
  • J. SHERMA (Easton, PA, USA)
  • M. WAKSMUNDZKA-HAJNOS (Lublin, Poland)
  • D. WEDGE (Mississippi, USA)

A. Kakuk - Managing Editor, Akadémiai Kiadó
Address: P.O. Box 245. H-1519 Budapest, Hungary
Fax: +36 1 464 82 95
E-mail: jpc@akkrt.hu

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences

 

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Apr 2021 7 0 0
May 2021 1 0 0
Jun 2021 8 0 0
Jul 2021 8 0 0
Aug 2021 11 0 0
Sep 2021 11 0 0
Oct 2021 0 0 0