A densitometric thin-layer chromatographic method has been established for quantitative determination of β-sitosterol in pumpkin seed oil (
L.). Chromatography was performed on silica gel 60 F
TLC plates, with toluene-ethyl acetate-glacial acetic acid 15:4:1 (
) as mobile phase. Densitometric quantitation was performed at 525 nm. The method was validated by determination of linearity (15–750 μg mL
), precision (RSD = 2.36%), and limits of detection (LOD = 0.65 μg per band) and quantification (LOQ = 1.99 μg per band). Average recovery from the pharmaceutical preparation was 96.15%. The method enables simple, rapid, and precise quantitative determination of β-sitosterol in pharmaceutical preparations and can be used for routine quality control.
(Ed.) Podstawy farmakologii: dla lekarzy, farmaceutów i studentów medycyny, Volumed, Wrocław, 1996.
I. Kreft, V. Stibilj
, Eur. Food Res. Technol.