A simple, sensitive, and rapid high-performance thin layer chromatographic (HPTLC) method has been established for estimation of piperine in commercial Ayurvedic formulations and in the fruits of Piper nigrum Linn, and Piper longum Linn. Chromatography was performed on aluminum foil HPTLC plates coated with 0.2 mm layers of silica gel F 254 , with hexane-acetone 6.5: 3.5 ( v/v ) as mobile phase. The development distance was 76 mm, the temperature 25 ± 5°C, and the chamber was saturated for 5 min. Piperine was quantified at 340 nm, its wavelength of maximum absorbance. Under the conditions used the R F of piperine was 0.33 and the limit of detection (LOD) was 4 ng per zone. The calibration plot was linear in the range of 10 to 60 ng per zone with a correlation coefficient of 0.9996. Recovery was in the range 98.76 to 100.70%. This HPTLC method was found to be reproducible, accurate, and precise and could be used to detect piperine at nanogram levels. The method is a very simple and cost-effective means of quantitative estimation of piperine in Ayurvedic formulations.
-
B. Yin Quan , Epilepsy 24 (1983) 177–178.
Yin Quan B. , '' (1983 ) 24 Epilepsy : 177 -178 .
-
R.K. Johari and U. Zutshi , J. Ethnopharmacol. 37 (1992) 85–91.
Zutshi U. , '' (1992 ) 37 J. Ethnopharmacol. : 85 -91 .
-
V.V. Sivarajan and I. Balachandra , Ayurvedic Drugs and their Plant Sources, Janapath, New Delhi, India, 1994.
-
A. Khajuria, N. Thuso, U. Zutshi , and K.L. Bedi , Indian Drugs 34 (1997) 557–563.
Bedi K.L. , '' (1997 ) 34 Indian Drugs : 557 -563 .
-
A. Khajuria, N. Thuso, U. Zutshi , and K.L. Bedi , Mol. Cell Biochem. 189 (1998) 113–118.
Bedi K.L. , '' (1998 ) 189 Mol. Cell Biochem. : 113 -118 .
-
J. Szolcsányi , Neurosci. Lett. 4 (1983) 03–86.
Szolcsányi J. , '' (1983 ) 4 Neurosci. Lett. : 03 -86 .
-
E.B. Lee, K.H. Shin , and W.S. Woo , Arch. Pharmacol. Res. 7 (1984) 127–132.
Woo W.S. , '' (1984 ) 7 Arch. Pharmacol. Res. : 127 -132 .
-
I.B. Koul , and A. Kapil , Planta Med. 59 (1993) 413–417.
Kapil A. , '' (1993 ) 59 Planta Med. : 413 -417 .
-
A.C. Reddy , and B.R. Lokesh , Mol. Cell Biochem. 111 (1992) 117–124.
Lokesh B.R. , '' (1992 ) 111 Mol. Cell Biochem. : 117 -124 .
-
R.K. Reen, F. Wiebel , and J. Singh , J. Ethnopharmacol. 58 (1997) 165–173.
Singh J. , '' (1997 ) 58 J. Ethnopharmacol. : 165 -173 .
-
Findings of Regional Research Laboratory (CSIR), Jammu.
-
C.K. Attal, R.K. Dubey , and J. Singh , J. Pharmacol. Exp. Ther. 232 (1985) 258–262.
Singh J. , '' (1985 ) 232 J. Pharmacol. Exp. Ther. : 258 -262 .
-
R.R. Dalvi and P.S. Dalvi , Drug Chem. Toxicol. 14 (1991) 219–229.
Dalvi P.S. , '' (1991 ) 14 Drug Chem. Toxicol. : 219 -229 .
-
J.T. Traxler , J. Agric. Food Chem. 19 (1971) 1135–1138.
Traxler J.T. , '' (1971 ) 19 J. Agric. Food Chem. : 1135 -1138 .
-
A. Mensah , Phytochemistry 16 (1977) 483–485.
Mensah A. , '' (1977 ) 16 Phytochemistry : 483 -485 .
-
M. Verzele, P. Muussche , and S.A. Qureshi , J. Chromatogr. 172 (1979) 493–497.
Qureshi S.A. , '' (1979 ) 172 J. Chromatogr. : 493 -497 .
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| Journal of Planar Chromatography - Modern TLC | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1988 |
| Volumes per Year |
1 |
| Issues per Year |
6 |
| Founder | Akadémiai Kiadó |
| Founder's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Publisher | Akadémiai Kiadó Springer Nature Switzerland AG |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 0933-4173 (Print) |
| ISSN | 1789-0993 (Online) |
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