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Barbara Sparzak Medical University of Gdańsk Department of Pharmacognosy with the Medicinal Plants Garden Hallera 107 80-416 Gdańsk Poland

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Mirosława Krauze-Baranowska Medical University of Gdańsk Department of Pharmacognosy with the Medicinal Plants Garden Hallera 107 80-416 Gdańsk Poland

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Loretta Pobłocka-Olech Medical University of Gdańsk Department of Pharmacognosy with the Medicinal Plants Garden Hallera 107 80-416 Gdańsk Poland

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Densitometric HPTLC has been used for determination of catechins in shoot and callus cultures of two species from the genus Phyllanthus, P. juglandifolius and P. grandifolius . The catechin composition of intact P. juglandifolius (leaves and stems) was also compared with that of biomass obtained by biotechnological methods. A mixture of the standards (−)-epicatechin, (+)-catechin, (−)-epigallocatechin, (−)-gallocatechin, (−)-catechin gallate, and (−)-epicatechin gallate was separated on C 18 plates, with methanol-water-formic acid 30:70:6 ( v/v ) as mobile phase, in an automated development chamber at 75% humidity. The catechins were detected at 440 nm after derivatization with diazotized sulfanilic acid. The total catechin content of the plant material was calculated on the basis of the amount of (+)-catechin. The method was validated for precision and repeatability. (−)-Epicatechin, (+)-catechin, (−)-epigallocatechin, and (−)-gallocatechin were shown to be present in all shoot and callus cultures of the species mentioned above and in the leaves of intact P. juglandifolius plants. Amounts of catechins were similar in all the samples — from 2.39 mg g −1 in the leaves of P. juglandifolius to 1.94–1.68 mg g −1 in the shoot cultures of both species.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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