The acidic visualization methods most often used for steroids have been optimized and compared. Sulfuric acid at three different concentrations, phosphomolybdic acid, and phosphoric acid, with different heating temperatures for different times, have been examined as reagents for visualization of seven steroids separated by OPLC on HPTLC silica gel as adsorbent. For sulfuric and phosphoric acids the chromatograms were evaluated by inspection under long-wavelength UV light (366 nm). For phosphomolybdic acid the chromatograms were evaluated in daylight. It was found that visualization at higher temperatures for shorter periods usually results in greater sensitivity, although heating for longer periods at lower temperatures results in a more stable, robust visualization.
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| Journal of Planar Chromatography - Modern TLC | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1988 |
| Volumes per Year |
1 |
| Issues per Year |
6 |
| Founder | Akadémiai Kiadó |
| Founder's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Publisher | Akadémiai Kiadó Springer Nature Switzerland AG |
| Publisher's Address |
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| ISSN | 0933-4173 (Print) |
| ISSN | 1789-0993 (Online) |
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