The role of the mobile phase in controlling selectivity for adsorption chromatography — with either thin-layer plates or columns — is reviewed and expanded. A primary factor responsible for these differences in selectivity is the localization of solvent molecules at the surface of the column packing. The hydrogen-bond basicity of the solvent plays a further, less significant role in determining solvent selectivity.
L.R. Snyder, J.J. Kirkland
, Practical HPLC Method Development, 2nd ed., Wiley-Interscience, New York, 1997.
Glajch J.L., '', in Practical HPLC Method Development, (1997) -.