JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 21: Issue 5
Determination of flavonoids in tea and Rooibos extracts by TLC and HPLC
Authors:
Magdalena Ligor Nicolaus Copernicus University Faculty of Chemistry 7 Gagarin St 87 100 Toruń Poland

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Olga Kornyšova Vytautas Magnus University Department of Chemistry Vileikos 8 Kaunas 44404 Lithuania

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Audrius Maruška Vytautas Magnus University Department of Chemistry Vileikos 8 Kaunas 44404 Lithuania

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Bogusław Buszewski Nicolaus Copernicus University Faculty of Chemistry 7 Gagarin St 87 100 Toruń Poland

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Pages:
355–360
Online Publication Date:
22 Oct 2008
Publication Date:
01 Oct 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.21.2008.5.7
Keywords:
TLC; Flavonoids; Black tea; Green tea; Rooibos
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In this study thin-layer chromatography (TLC) was used for analysis of the constituents of extracts of tea ( Camellia sinensis L.) and Rooibos ( Aspalathus linearis ) leaves. In particular, flavonoids, a group of phenolic compounds, for example myrecetin, rutin, catechin, quercetin, and kaempferol were analyzed. Extracts of various types of tea, for example black, green, and Rooibos herbal tea (called red tea) were analyzed. The efficiency of extraction of flavonoids from plant material by classical liquid extraction (LE) and supercritical-fluid extraction (SFE) was also compared. Recoveries of individual flavonoids were approximately ten times higher after use of solvent extraction. Separation of flavonoids from tea extracts on the basis of their polarities was optimized by selection of solvents, stationary phases, and chromatographic conditions. For final analyses glass-backed silica gel 60 F 254 plates and the mobile phase acetone-chloroform-water 80:20:10 ( v / v ) were used. UV detection of TLC chromatograms (254 and 366 nm) was used. Better detection of the flavonoids was achieved at 366 nm. The concentration of some important flavonoids in real tea samples was obtained by high-performance liquid chromatography (HPLC).

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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