JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 21: Issue 5
Fully on-line hyphenation of an experimental OPLC separation unit with diode-array detection and mass-spectrometry (OPLC-DAD-MS) for analysis of xanthine compounds
Authors:
Emil Mincsovics

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Katalin Pápai Semmelweis University Department of Pharmaceutics Hőgyes Endre Street 7 1092 Budapest Hungary

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Krisztina Ludányi Semmelweis University Department of Pharmaceutics Hőgyes Endre Street 7 1092 Budapest Hungary

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Ádám Dávid Semmelweis University Department of Pharmaceutics Hőgyes Endre Street 7 1092 Budapest Hungary

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Marianna Budai Semmelweis University Department of Pharmaceutics Hőgyes Endre Street 7 1092 Budapest Hungary

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István Antal Semmelweis University Department of Pharmaceutics Hőgyes Endre Street 7 1092 Budapest Hungary

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Imre Klebovich Semmelweis University Department of Pharmaceutics Hőgyes Endre Street 7 1092 Budapest Hungary

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Pages:
361–366
Online Publication Date:
22 Oct 2008
Publication Date:
01 Oct 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.21.2008.5.8
Keywords:
Overpressured-layer chromatography (OPLC); OPLC Separation Unit 100 (OSU 100); Fully on-line multiple hyphenation; OPLC-DAD-ESI-MS; Xanthines (caffeine, theophylline, theobromine); Green tea
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The newly developed experimental OPLC separation unit 100 (OSU 100) has been used for fully on-line multiple hyphenation using the one-channel flowing eluent wall (FEW) arrangement at the inlet. OSU 100-UV and OSU 100-DAD-ESI-MS with manual injector or autosampler were used to test the connected systems (OPLC-UV; OPLC-DAD-ESI-MS), using xanthine standards (caffeine, theophylline, theobromine) and green tea leaf extract as model compounds. The mobile phase chloroform-trifluoroacetic acid-acetonitrile-methanol, 76:4:6.67:13.33 ( v / v ) is suitable for rapid and selective separation, DAD, and ESI-MS of xanthines. The detection sensitivity of OPLC-UV or OPLC-DAD was increased by hyphenation with ESI-MS coupled in series. In contrast with DAD, the extracted ion chromatogram ( m / z = 181.1 Da) after background subtraction yields readily measurable peaks from the small amounts of theophylline and theobromine in tea leaf extract. Analysis of xanthines was achieved within 10 min with less than 2 mL mobile phase. Owing to the openable adsorbent layer in OPLC the bed conditions can, moreover, be checked off-line after single or multiple fully online separations.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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