JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 21: Issue 5
Comparison of two different plunger geometries for HPTLC-MS coupling via an extractor-based interface
Authors:
Gertrud Morlock University of Hohenheim Institute of Food Chemistry Garbenstrasse 28 70599 Stuttgart Germany

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Ute Jautz University of Hohenheim Institute of Food Science and Biotechnology, Department of Meat Technology Garbenstrasse 21 70593 Stuttgart Germany

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Pages:
367–371
Online Publication Date:
22 Oct 2008
Publication Date:
01 Oct 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.21.2008.5.9
Keywords:
Interface for online extraction; Plunger geometry; HPTLC-MS; Heterocyclic aromatic amines
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Plungers with two different cutting edge geometries were compared for HPTLC-MS coupling via an extractor-based interface. Their performance was investigated on the example of the analysis of harmane and Glu-P-1, both heterocyclic aromatic amines. The plungers resulted in similar performance with regard to the delay time between switching the valve and recording the signal, the system pressure, the short negative baseline amplitude at the signal start of the elution peak, the time for complete extraction (elution peak width), and blank plate signals. The mean elution peak widths were 0.51 min for the oval plunger and 0.65 min for the circular plunger. The mean repeatability of the SIM signal for 4 ng per band harmane on three plates ( RSD , %, n = 3 plate means) was 13.3% by use of the circular plunger and 13.6% by use of the oval plunger. This was comparable with results from HPLC-MS, for which repeatability of 9.5% ( n = 6 injections) was obtained. The plunger with the oval geometry, specially designed for band-shaped zones, was better for precise positioning on the substance band of interest and for avoiding co-detection of adjacent zones. It was demonstrated that the oval plunger was more selective than the circular plunger and produced more intense signals for adjacent zones. On the other hand the circular plunger was easier to position on the zone and, for non-adjacent zones, resulted in generally higher signal intensity, because of its larger cross-section.

  • A. Alpmann, G. Morlock , Anal. Bioanal. Chem. 386 (2006) 1543–1551.

    Morlock G. , '' (2006 ) 386 Anal. Bioanal. Chem. : 1543 -1551 .

  • H. Luftmann , Anal. Bioanal. Chem. 378 (2004) 964–968.

    Luftmann H. , '' (2004 ) 378 Anal. Bioanal. Chem. : 964 -968 .

  • M. Prosek, L. Milivojevic, M. Krizman, and M. Fir , J. Planar Chromatogr. 17 (2004) 420–423.

    Fir M. , '' (2004 ) 17 J. Planar Chromatogr. : 420 -423 .

  • A. Crecelius, M.R. Clench, D.S. Richards, D. Evason, and V. Parr , J. Chromatogr. Sci. 40 (2002) 614–620.

    Parr V. , '' (2002 ) 40 J. Chromatogr. Sci. : 614 -620 .

  • A. Orinak, G. Verlng, H.F. Arlinghaus, J.T. Andersson, L. Halas, and R. Orinakova , J. Planar Chromatogr. 18 (2005) 44–50.

    Orinakova R. , '' (2005 ) 18 J. Planar Chromatogr. : 44 -50 .

  • G.J. Van Berkel, V. Kertesz , Anal. Chem. 78 (2006) 4938–4944.

    Kertesz V. , '' (2006 ) 78 Anal. Chem. : 4938 -4944 .

  • G. Morlock, Y. Ueda , J. Chromatogr. A 1143 (2007) 243–251.

    Ueda Y. , '' (2007 ) 1143 J. Chromatogr. A : 243 -251 .

  • E. Dytkiewitz, G. Morlock , J. Assoc. Offic. Anal. Chem. (2008) in print.

  • U. Jautz, G. Morlock , J. Chromatogr. A 1128 (2006) 244–250.

    Morlock G. , '' (2006 ) 1128 J. Chromatogr. A : 244 -250 .

  • A. Alpmann, G. Morlock , J. Sep. Sci. 31 (2008) 71–77.

    Morlock G. , '' (2008 ) 31 J. Sep. Sci. : 71 -77 .

  • H. Luftmann, M. Aranda, and G. Morlock , Rapid Commun. Mass Spectrom. 21 (2007) 3772–3776.

    Morlock G. , '' (2007 ) 21 Rapid Commun. Mass Spectrom. : 3772 -3776 .

  • M. Aranda, G. Morlock , Rapid Commun. Mass Spectrom. 21 (2007) 1297–1303.

    Morlock G. , '' (2007 ) 21 Rapid Commun. Mass Spectrom. : 1297 -1303 .

  • G. Morlock, A. Nedele, W. Schwack , Proceedings of Dt. Lebensmittelchemikertag, Hamburg, 2005, 513–516.

  • G. Morlock, W. Schwack , Anal. Bioanal. Chem. 385 (2006) 586–595.

    Schwack W. , '' (2006 ) 385 Anal. Bioanal. Chem. : 586 -595 .

  • M. Aranda, G. Morlock , J. Chromatogr. A 1131 (2006) 253–260.

    Morlock G. , '' (2006 ) 1131 J. Chromatogr. A : 253 -260 .

  • U. Jautz, G. Morlock , Anal. Bioanal. Chem. 387 (2007) 1083–1093.

    Morlock G. , '' (2007 ) 387 Anal. Bioanal. Chem. : 1083 -1093 .

  • M. Aranda, G. Morlock , J. Chromatogr. Sci. 45 (2007) 251–255.

    Morlock G. , '' (2007 ) 45 J. Chromatogr. Sci. : 251 -255 .
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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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