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  • 1 Zweckverband Landeswasserversorgung, Betriebs-und Forschungslaboratorium Am Spitzigen Berg 1 89129 Langenau Germany
  • 2 Aalen University of Applied Sciences Beethovenstraße 1 73430 Aalen Germany
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Combination of HPTLC with the bioluminescence test using Vibrio fischeri enables effective screening for bioactive substances in unknown samples. This paper describes several applications in environmental testing using bioluminescence inhibition in HPTLC. The samples were extracted by solid-phase extraction or applied directly to the TLC plate. After chromatography of the samples the plates were immersed in a suspension of bioluminescence bacteria. The bioactive substances were identified as dark or light zones in comparison with the background of the plate. By the use of this method several types of sports field granule were compared for their toxicity toward Vibrio fischeri . It was also possible to compare the bioactivity of expressway waste water or landfill leachate by use of the method. To evaluate the digital images taken by a CCD camera, inhibition chromatograms over the developed sample tracks were created.

  • C. Weins and M. Oehme-Peter , J. Verbraucherschutz und Lebensmittelsicherheit 1 (2006) 332–344.

    Oehme-Peter M. , '' (2006 ) 1 J. Verbraucherschutz und Lebensmittelsicherheit : 332 -344.

  • T. Reemtsma, A. Putschew and M. Jekel , Waste Manag. 19 (1999) 181–188.

    Jekel M. , '' (1999 ) 19 Waste Manag : 181 -188.

  • T. Reemtsma , Anal. Chim. Acta 426 (2001) 279–287.

    Reemtsma T. , '' (2001 ) 426 Anal. Chim. Acta : 279 -287.

  • F. Geike , J. Chromatogr. 63 (1971) 343–347.

    Geike F. , '' (1971 ) 63 J. Chromatogr. : 343 -347.

  • C. Weins and H. Jork , J. Chromatogr. A 750 (1996) 403–407

    Jork H. , '' (1996 ) 750 J. Chromatogr. A : 403 -407.

  • A. Marston, J. Kissling and K. Hostettmann , Phytochem. Anal. 13 (2002) 51–54.

    Hostettmann K. , '' (2002 ) 13 Phytochem. Anal. : 51 -54.

  • G. Eberz, H. Rast, K. Burger, W. Kreiss and C. Weisemann , Chromatographia 43 (1996) 5–9

    Weisemann C. , '' (1996 ) 43 Chromatographia : 5 -9.

  • S. M. Verbitski, G. T. Gourdin, L. M. Ikenouye and J. D. McChesney , American Biotechnology Laboratory 24 (2006) 40–42.

    McChesney J. D. , '' (2006 ) 24 American Biotechnology Laboratory : 40 -42.

  • N. Reineke , Dissertation, University of Hamburg, (2003).

  • DIN 38 412-L34/L341

  • EN ISO 11348-3:s1998

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