View More View Less
  • 1 Medical University Department of Inorganic Chemistry 20-081 Lublin Poland
  • 2 Medical University Department of Pharmacognosy 20-093 Lublin Poland
  • 3 Medical University Department of Pharmaceutical Botany 20-093 Lublin Poland
Restricted access

Coumarins are interesting group of natural compounds, because of their biological and pharmacological activity, and are widely investigated. They are normally found in complex mixtures, e.g. plant extracts, and are difficult to separate in one chromatographic run. Mixtures of coumarins have been separated by two-dimensional thin-layer chromatography on CN-silica plates by use of aqueous and nonaqueous mobile phases. Complete separation was also achieved by use of graft thin-layer chromatography on connected layers — silica with RP-18W or CN-silica with silica. The systems characterized by the best efficiency and selectivity were used for separation of coumarin fractions from extracts of selected Apiaceae plants. These orthogonal systems were used for quantitative analysis of selected coumarins. The results obtained show two dimensional thin-layer chromatography is useful tool for quantification of some furanocoumarins in plant extracts. The best results were obtained on connected silica and octadecyl silica layers.

  • A. Estevez-Braun and A.G. Gonzales , Nat. Prod. Rep. 14 (1997) 465–475.

    Gonzales A.G. , '' (1997 ) 14 Nat. Prod. Rep. : 465 -475.

  • K. Głowniak , Investigation and isolation of coumarin derivatives from Polish plant material (in Polish), Dissertation, Medical University, Lublin, Poland, 1988.

    Głowniak K. , '', in Investigation and isolation of coumarin derivatives from Polish plant material (in Polish) , (1988 ) -.

    • Search Google Scholar
  • W. Cisowski , Herba Polonica 29 (1983) 301–318 (in Polish).

    Cisowski W. , '' (1983 ) 29 Herba Polonica : 301 -318.

  • J.R.S Hoult and M. Paya , Gen. Pharmac. 27 (1996) 713–722.

    Paya M. , '' (1996 ) 27 Gen. Pharmac. : 713 -722.

  • F. Hadaček, C. Müller, A. Werner, H. Greger and P. Proksh , J. Chem. Ecol. 20 (1994) 2035–2054.

    Proksh P. , '' (1994 ) 20 J. Chem. Ecol. : 2035 -2054.

  • T. Wolski, J. Gliński, K. Buczek and A. Wolska , Herba Polonica 42 (1996) 168–173 (in Polish).

    Wolska A. , '' (1996 ) 42 Herba Polonica : 168 -173.

  • M. Waksmundzka-Hajnos, A. Petruczynik, M.Ł. Hajnos, T. Tuzimski, A. Hawrył , and A. Bogucka-Kocka , J. Chromatogr. Sci. 44 (2006) 510–517.

    Bogucka-Kocka A. , '' (2006 ) 44 J. Chromatogr. Sci. : 510 -517.

  • Z. Jerzmanowska , Plant substances. Methods of Isolation, PWN, Warszawa, 1967 (in Polish).

    Jerzmanowska Z. , '', in Plant substances , (1967 ) -.

  • M. Waksmundzka-Hajnos and T. Wawrzynowicz , J. Planar Chromatogr. 5 (1992) 168–174.

    Wawrzynowicz T. , '' (1992 ) 5 J. Planar Chromatogr. : 168 -174.

  • M. Bartnik, K. Głowniak, A. Maciag and M. Hajnos , J. Planar Chromatogr. 18 (2005) 244–248.

    Hajnos M. , '' (2005 ) 18 J. Planar Chromatogr. : 244 -248.

  • M.A. Hawrył, E. Soczewiński and T.H. Dzido , J. Chromatogr. A 366 (2000) 75–81.

    Dzido T.H. , '' (2000 ) 366 J. Chromatogr. A : 75 -81.

  • Ł. Cieśla, A. Petruczynik, M.Ł. Hajnos, A. Bogucka-Kocka and M. Waksmundzka-Hajnos , J. Planar Chromatogr. 21 (2008) 237–241.

    Waksmundzka-Hajnos M. , '' (2008 ) 21 J. Planar Chromatogr. : 237 -241.

  • Sz. Nyiredy , Planar Chromatography — A Retrospective View for the Third Millennium, Springer, Budapest, 2001.

    Nyiredy Sz. , '', in Planar Chromatography — A Retrospective View for the Third Millennium , (2001 ) -.

    • Search Google Scholar
  • C.F. Poole, S.K. Poole, W.P.N. Fernando, T.A. Dean, H.D. Ahmed and J.A. Berndt , J. Planar Chromatogr. 2 (1989) 336–345.

    Berndt J.A. , '' (1989 ) 2 J. Planar Chromatogr. : 336 -345.

  • B. Spangenberg , J. Planar Chromatogr. 19 (2006) 332–341.

    Spangenberg B. , '' (2006 ) 19 J. Planar Chromatogr. : 332 -341.

  • E. Nováková , J. Planar Chromatogr. 13 (2000) 221–225.

    Nováková E. , '' (2000 ) 13 J. Planar Chromatogr. : 221 -225.

  • T. Tuzimski , J. Planar Chromatogr. 20 (2007) 13–18.

    Tuzimski T. , '' (2007 ) 20 J. Planar Chromatogr. : 13 -18.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 1 1
Jul 2020 2 0 0
Aug 2020 1 0 0
Sep 2020 3 0 0
Oct 2020 1 0 0
Nov 2020 7 0 0
Dec 2020 0 0 0