JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 22: Issue 1
Bioactivity-based analysis of sunscreens using the luminescent bacteria Vibrio fischeri
Authors:
Vera BaumgartnerKantonales Laboratorium Basel-Stadt Department Non-Food Kannenfeldstrasse 2 4012 Basel Switzerland

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Christopher HohlKantonales Laboratorium Basel-Stadt Department Non-Food Kannenfeldstrasse 2 4012 Basel Switzerland

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Urs HauriKantonales Laboratorium Basel-Stadt Department Non-Food Kannenfeldstrasse 2 4012 Basel Switzerland

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Pages:
19–23
Online Publication Date:
20 Feb 2009
Publication Date:
01 Feb 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.22.2009.1.4
Keywords:
Bioactivity; Vibriofischeri ; HPTLC; Sunscreen; UV filters; Photodegradation
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Sunscreen products are meant to protect people from damaging UVA and UVB radiation. However, in some formulations the UV filters they contain can react and form many photodegradation products. Their potential toxicity has not yet been investigated. In this study effect-specific analysis has been used to evaluate the bioactivity of photodegradation products in sunscreens. HPTLC-bioluminescence coupling with the luminescent bacterium Vibrio fischeri was used. Problems in method development were because of the sensitivity of the bacteria and the wettability of HPTLC plates. A separation system using HPTLC LiChrospher plates and automated multiple development (AMD) with tert -butyl methyl ether- n -hexane was chosen. Detection was by UV in addition to Vibrio fischeri . First, biodetection was performed on pure standard solutions of the UV filters. UV filters with molecular weight >400 had no bioactivity; these included all newer UV filters (not in use before 1998). Five commercially available sunscreens with different UV filter combinations were then analyzed. They were irradiated on microscope slides with artificial light and natural sunlight and on the skin with natural sunlight. For extraction, a mixture of ethanol and acetone was used. The bioactivity which can be indicative of (cyto)toxic effects of the photodegradation products was higher than that of the corresponding UV filter. In comparison of HPLC-DAD and LC-MS with detection with Vibrio fischeri , a high signal in chemical-physical detection did not always correspond to high bioactivity, and vice versa. It was shown that biodetection with Vibrio fischeri was a suitable method for examination of photodegradation products in sunscreens, making this bioassay a useful addition to conventional analytical methods.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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