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Kunbo Wang Hunan Agricultural University Key Laboratory of Tea Science of the Ministry of Education Furong District Changsha Hunan 410128 People’s Republic of China

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Zhonghua Liu Hunan Agricultural University Key Laboratory of Tea Science of the Ministry of Education Furong District Changsha Hunan 410128 People’s Republic of China

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Jian-an Huang Hunan Agricultural University Key Laboratory of Tea Science of the Ministry of Education Furong District Changsha Hunan 410128 People’s Republic of China

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Donghe Fu Hunan Agricultural University Key Laboratory of Tea Science of the Ministry of Education Furong District Changsha Hunan 410128 People’s Republic of China

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Fang Liu Hunan Agricultural University Key Laboratory of Tea Science of the Ministry of Education Furong District Changsha Hunan 410128 People’s Republic of China

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Yushun Gong Hunan Agricultural University Key Laboratory of Tea Science of the Ministry of Education Furong District Changsha Hunan 410128 People’s Republic of China

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Xiaosong Wu Hunan Agricultural University Key Laboratory of Tea Science of the Ministry of Education Furong District Changsha Hunan 410128 People’s Republic of China

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The potential of polyamide as adsorbent for separation of nine compounds — (±)-catechin (DL-C), (−)-epicatechin (EC), (−)-epigallocatechin (EGC), (−)-epicatechin gallate (ECg), (−)-epigallocatechin gallate (EGCg), theaflavin (TF1), theaflavin 3-gallate (TF2A), theaflavin 3′-gallate (TF2B), and theaflavin 3,3′-digallate (TF3) — was studied. Polyamide TLC plates dried with a hair dryer, application as bands, and development in a horizontal chamber gave the best results. Detection was performed using iron(III) chloride-ethanol reagent. Twofold development with chloroform-methanol 2:3 ( v/v ) as mobile phase resulted in the separation of TF1, TF2A, TF2B, and TF3 from all the other compounds. EGCg, ECg, and EGC were separated but EC and DL-C were not separated from each other. The best mobile phase for each of the compounds is reported. The best separation of the five main catechins (EC, DL-C, EGC, ECg, and EGCg) was achieved by use of n -butanol-acetone-acetic acid 5:5:3 ( v/v ). In addition, separation of the flavonols myricetin, quercetin, kaempferol, and rutin and the phenolic acids gallic acid, chlorogenic acid, and caffeic acid was achieved by twofold development with chloroform-methanol 2:3 ( v/v ). The applicability of the method was checked by screening of extracts of green, black, oolong, and pu-erh tea.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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